JOURNAL OF THROMBOSIS AND HAEMOSTASIS

Association of fibrinogen α, fibrinogen γ´, and sialylated fibrinogen with development of ischemic stroke in patients with recently diagnosed type 2 diabetes
Daugaard N, Bladbjerg EM, Svane HML, Thomsen RW, Nielsen JS, Palarasah Y, de Maat MPM and Münster AB
Stroke is a major cause of death globally, especially in type 2 diabetes (T2D) patients. Fibrinogen is known to predict stroke risk, but fibrinogen is a highly variable protein and we hypothesized that fibrinogen variants can improve stroke prediction.
"Estimation of gestational age-specific reference intervals for coagulation assays in a neonatal intensive care unit using real-world data": comment from Karlaftis et al
Karlaftis V, Attard C, Ignjatovic V and Monagle P
Implementation of the ISTH Framework for Professional Conduct
Scarlatescu E, Riva N, Casanegra AI, Rothschild C, Orsi FA, Ariëns RAS and
Thrombin reveals new faces with a tiny trick
De Cristofaro R
From bench to bedside: the role of tailoring fibrinolysis in pulmonary embolism
Maginot ER, Henry R and Barrett CD
"Estimation of gestational age-specific reference intervals for coagulation assays in a neonatal intensive care unit using real-world data": reply
Lalos N, Vesoulis Z, Maucione C and Spies NC
The critical role of platelet adenylyl cyclase 6 in haemostasis and thrombosis
Webb BA, Cheah LT, Khalil JS, Hindle MS, McKay M, Turner NA, Kearney MT, Ariens RAS, Duval C and Naseem KM
Platelet activation is constrained by endothelial-derived prostacyclin (PGI) through cyclic adenosine-5'-monophosphate (cAMP) signalling involving multiple isoforms of adenylyl cyclase (AC). The roles of specific AC isoforms in controlling haemostasis remain unclear and require clarification.
Discerning Specific Thrombolytic Activities and Blood Clot Degradomes of Diverse Snake Venoms with Untargeted Peptidomics
Smith CF, Baldé MA, French S, Modahl CM, Bahrabadi L, Amponsah-Asamoah M, Larson KY, Maroney SP, Ceja Galindo D, Millimouno M, Camara N, Benjamin J, Brandehoff NP, McCabe MC, Cohen MJ, Jackson K, Baldé C, Castoe TA, Mackessy SP, Hansen KC and Saviola AJ
Many snake venoms have been shown to possess thrombolytic activity. However, it remains unclear if actions on other clot-stabilizing proteins beyond fibrin chains contribute significantly to venom-induced thrombolysis because the clot-wide targets of venom proteases and the mechanisms responsible for thrombolysis are not well understood.
Consensus protocol for platelet desialylation (β-galactose exposure) quantification using lectins by flow cytometry: Communication from the ISTH SSC Subcommittee on Platelet Physiology
Kauskot A, Ramstrom S, Nipoti T and van der Wal DE
Platelets contain many heterogeneous carbohydrates (glycans): often capped by sialic acid. The removal of sialic acid (desialylation) is important for platelet function and clearance, leading to novel diagnostic markers. Platelet desialylation can be easily measured using inexpensive, user-friendly lectins, and flow cytometry.
Plasminogen and plasmin induce specialized pro-resolving mediators and promote efferocytosis via 5-lipoxygenase
Perucci LO, Carneiro FS, Souza JC, Grossi LC, Souza JAM, Zaidan I, Cardoso C, Lara ES, Felix FB, Baik N, Tavares LP, Soriani FM, Teixeira MM, Parmer RJ, Miles LA and Sousa LP
The plasminogen (Plg)/plasmin (Pla) system has been recognized for its pro-resolving actions, such as promoting efferocytosis. However, the role of specialized pro-resolving mediators (SPMs) in these Plg/Pla effects remains unexplored.
Thrombotic risk determined by CREB3L1 variants in a population-based cohort study: linkage disequilibrium with prothrombin mutation
Manderstedt E, Halldén C, Lind-Halldén C, Elf J, Svensson PJ, Engström G, Melander O, Baras A, Lotta LA, Zöller B and
Effect of direct oral anticoagulants in cirrhosis: an in vitro study
Pereira Portela C, Calderara DB, Mdawar-Bailly E, Aliotta A, Veuthey L, Gautier LA, Moradpour D, Fraga M, Zermatten MG and Alberio L
Cirrhosis is associated with a procoagulant state that may worsen disease evolution. Anticoagulation could be of particular interest in these patients. However, evidence on the use of DOAC in patients with cirrhosis is limited. Our aim was to explore the in vitro effect of DOAC on thrombin generation (TG) in plasma from patients with cirrhosis compared to healthy controls.
Causal insights into the role of metabolites in venous thromboembolism pathogenesis: A metabolome-wide mendelian randomization study
Hu W, Bei Y, Chen G, Xu J, Yang M, Yu L, He W, Hu Y, Mao F, Chen S, Xu D and Dai H
Venous thromboembolism (VTE) is a significant global health burden, and metabolic alterations play a key role in its pathogenesis. However, previous studies have been constrained by several limitations, hindering clarification of the causal role of metabolites.
Refractory autoimmune heparin-induced thrombocytopenia following cardiac surgery
Zlamal J, Bohnert BN, Althaus K, Tilly R, Häberle H, Schlensak C, Rosenberger P and Bakchoul T
Autoimmune heparin-induced thrombocytopenia (aHIT) is a severe subtype of HIT characterized by persistent thrombocytopenia and prothrombotic condition even though anticoagulation with heparin has been discontinued. Here we report on a patient with a previous history of aHIT where re-exposure to heparin during cardiac surgery resulted in recurrent aHIT with pulmonary embolism. Alternative anticoagulants as well as high dose intravenous immunoglobulin were ineffective, and only multiple cycles of therapeutic plasma exchange (TPE) restored platelet counts and prevented further thrombosis progression. The therapy was guided by an ex vivo model of anti-platelet factor 4 (PF4)-mediated thrombosis that showed accurate performance in predicting the clinical outcome. Most importantly, the ability to induce thrombus formation was mainly caused by anti-PF4 (heparin-independent) antibodies. Our paper provides the first description of recurrent aHIT with translational evidence that pathogenic heparin-independent anti-PF4 Abs can be specifically targeted by TPE, emphasizing the clinical use in refractory cases of aHIT.
Factor Activity Levels and Bleeding Scores in Hemophilia Carriers: Apparent Paradoxes
Puetz J
Multiple studies report abnormal bleeding in hemophilia carriers with normal factor activity levels. Other studies report the lack of abnormal bleeding in carriers with hemophilia. In addition, limited data suggests that the bleeding risk in carriers increases with age while at the same time, factor activity levels are rising. Potential explanations for these paradoxical findings include inadequate data, inadequate measures of bleeding, inadequate measures of factor activity, or uncharacterized biological modifiers.
Anticoagulation and Other Risk Factors for Clinically Relevant Bleeding after Medical Hospitalization: The Medical Inpatient Thrombosis and Hemostasis Study
Gergi M, Wilkinson K, Sparks A, Roetker NS, Al-Samkari H, Smith NL, Plante TB, Cushman M, Repp AB, Holmes CE, Martin K and Zakai NA
Clinically relevant Bleeding after discharge from a medical hospitalization is associated with increased morbidity and mortality. There is limited knowledge of the risk factors for this bleeding.
The Incidence and Risk Of Venous thromboembolism in patients with Active Malignancy and Isolated Superficial Venous Thrombosis: A systematic review and meta-analysis. (The IROVAM-iSVT review)
Bailey AJM, Luo OD, Zhou SQ and Wells PS
The management of cancer-associated isolated superficial venous thrombosis (iSVT) remains controversial as cancer patients are at higher risk of bleeding and venous thromboembolism (VTE).
"Reversal of direct oral anticoagulants: Guidance from the SSC of the ISTH": Reply
Levy JH, Shaw JR, Connors JM, Douketis J and Weitz JI
Maternal platelet-derived factors induce trophoblastic LAIR2 expression to promote trophoblast invasion and inhibit platelet activation at the fetal-maternal interface
Lyssy F, Forstner D, Guettler J, Kupper N, Ujčič K, Neuper L, Daxboeck C, El-Heliebi A, Kummer D, Krappinger JC, Vejzovic D, Rinner B, Cvirn G, Wernitznig S, Moser G, Valdes DS, Herse F, Höbler AL, Pollheimer J, James JL, Feichtinger J and Gauster M
During human placentation extravillous trophoblasts (EVTs), arising from cell column trophoblasts (CCT) invade the highly differentiated uterine mucosa, called decidua, where they erode blood vessels and replace vascular endothelial cells. Maternal platelets have been detected in intercellular gaps of CCTs but their physiological role remained unclear so far.
Trends in home treatment and early discharge of patients with low-risk pulmonary embolism
Farmakis IT, Christodoulou KC, Hobohm L, Giannakoulas G, Keller K, Lurz P, Valerio L, Barco S and Konstantinides SV
Recent guidelines recommend a strategy of home treatment or early discharge in low-risk pulmonary embolism (PE). Contemporary rates of the implementation of this approach in everyday clinical practice are unknown.
Enhancing hemostasis potency in hemophilia with a small interfering RNA targeting protein S
Eladnani RP, Schaeper U, Diab R, Aretz J, Vrotniakaite-Bajerciene K, Çaku S, Yasmin R, Li B, Reina Caro MD, Dames S, Eisermann M, Löffler K, Martinez A, de Laat B, Brodard J, Casini A, Kremer Hovinga JA, Allam R, Fernández JA, Griffin JH, Laffan MA, Majumder R, Ahnström J and Angelillo-Scherrer A
One hemophilia treatment concept focuses on rebalancing coagulation and anticoagulation to restore normal blood clotting. Targeting the coagulation regulator, protein S (PS), in hemophilia shows promise to increase the generation of thrombin-a critical enzyme in the clotting process.