Journal of Mechanics in Medicine and Biology

USING CONVOLUTIONAL NEURAL NETWORK-BASED SEGMENTATION FOR IMAGE-BASED COMPUTATIONAL FLUID DYNAMICS SIMULATIONS OF BRAIN ANEURYSMS: INITIAL EXPERIENCE IN AUTOMATED MODEL CREATION
Rezaeitaleshmahalleh M, Lyu Z, Mu N and Jiang J
"Image-based" computational fluid dynamics (CFD) simulations provide insights into each patient's hemodynamic environment. However, current standard procedures for creating CFD models start with manual segmentation and are time-consuming, hindering the clinical translation of image-based CFD simulations. This feasibility study adopts deep-learning-based image segmentation (hereafter referred to as Artificial Intelligence (AI) segmentation) to replace manual segmentation to accelerate CFD model creation. Two published convolutional neural network-based AI methods (MIScnn and DeepMedic) were selected to perform CFD model extraction from three-dimensional (3D) rotational angiography data containing intracranial aneurysms. In this study, aneurysm morphological and hemodynamic results using models generated by AI segmentation methods were compared with those obtained by two human users for the same data. Interclass coefficients (ICC), Bland-Altman plots, and Pearson's correlation coefficients (PCC) were combined to assess how well AI-generated CFD models were performed. We found that almost perfect agreement was obtained between the human and AI results for all eleven morphological and five out of eight hemodynamic parameters, while a moderate agreement was obtained from the remaining three hemodynamic parameters. Given this level of agreement, using AI segmentation to create CFD models is feasible, given more developments.
AGREEMENT OF HIP KINEMATICS BETWEEN TWO TRACKING MARKER CONFIGURATIONS USED WITH THE CODA PELVIS DURING ERGONOMIC ROOFING TASKS
Moore KD, Hawke AL, Carey RE, Wu JZ and Breloff SP
The anterior and posterior iliac spine markers frequently used to define the pelvis, are commonly occluded during three-dimensional (3D) motion capture. The occlusion of these markers leads to the use of various tracking marker configurations on the pelvis, which affect kinematic results. The purpose of this investigation was to examine the agreement of CODA pelvis kinematic results when two different tracking marker configurations were used during roofing tasks. 3D motion data were collected on seven male subjects while mimicking two roofing tasks. Hip joint angles (HJAs) were computed using the CODA pelvis with two different tracking marker configurations, the trochanter tracking method (TTM), and virtual pelvis tracking method (VPTM). Agreement between tracking marker configurations was assessed using cross-correlations, bivariate correlations, mean absolute differences (MADs), and Bland-Altman (BA) plots. The correlations displayed no time lag and strong agreement (all > 0.83) between the HJA from the VPTM and TTM, suggesting the timing occurrence of variables are comparable between the two tracking marker configurations. The MAD between the VPTM and TTM displayed magnitude differences, but most of the differences were within a clinically acceptable range. Caution should still be used when comparing kinematic results between various tracking marker configurations, as differences exist.
A COMPUTATIONAL ANALYSIS OF BONE FORMATION IN THE CRANIAL VAULT USING A COUPLED REACTION-DIFFUSION-STRAIN MODEL
Lee C, Richtsmeier JT and Kraft RH
Bones of the murine cranial vault are formed by differentiation of mesenchymal cells into osteoblasts, a process that is primarily understood to be controlled by a cascade of reactions between extracellular molecules and cells. We assume that the process can be modeled using Turing's reaction-diffusion equations, a mathematical model describing the pattern formation controlled by two interacting molecules (activator and inhibitor). In addition to the processes modeled by reaction-diffusion equations, we hypothesize that mechanical stimuli of the cells due to growth of the underlying brain contribute significantly to the process of cell differentiation in cranial vault development. Structural analysis of the surface of the brain was conducted to explore the effects of the mechanical strain on bone formation. We propose a mechanobiological model for the formation of cranial vault bones by coupling the reaction-diffusion model with structural mechanics. The mathematical formulation was solved using the finite volume method. The computational domain and model parameters are determined using a large collection of experimental data that provide precise three dimensional (3D) measures of murine cranial geometry and cranial vault bone formation for specific embryonic time points. The results of this study suggest that mechanical strain contributes information to specific aspects of bone formation. Our mechanobiological model predicts some key features of cranial vault bone formation that were verified by experimental observations including the relative location of ossification centers of individual vault bones, the pattern of cranial vault bone growth over time, and the position of cranial vault sutures.
A Newly Developed Tri-Leaflet Polymeric Heart Valve Prosthesis
Gaetano F, Bagnoli P, Zaffora A, Pandolfi A, Serrani M, Brubert J, Stasiak J, Moggridge GD and Costantino ML
The potential of polymeric heart valves (PHV) prostheses is to combine the hemodynamic performances of biological valves with the durability of mechanical valves. The aim of this work is to design and develop a new tri-leaflet prosthetic heart valve (HV) made from styrenic block copolymers. A computational finite element model was implemented to optimize the thickness of the leaflets, to improve PHV mechanical and hydrodynamic performances. Based on the model outcomes, 8 prototypes of the designed valve were produced and tested under continuous and pulsatile flow conditions, as prescribed by ISO 5840 Standard. A specially designed pulse duplicator allowed testing the PHVs at different flow rates and frequency conditions. All the PHVs met the requirements specified in ISO 5840 Standard in terms of both regurgitation and effective orifice area (EOA), demonstrating their potential as HV prostheses.
Quantifying Digit Force Vector Coordination during Precision Pinch
Marquardt TL and Li ZM
A methodology was established to investigate the contact mechanics of the thumb and the index finger at the digit-object interface during precision pinch. Two force/torque transducers were incorporated into an apparatus designed to overcome the thickness of each transducer and provide a flexible pinch span for digit placement and force application. To demonstrate the utility of the device, five subjects completed a pinch task with the pulps of their thumb and index finger. Inter-digit force vector coordination was quantified by examining the 1) force vector component magnitudes, 2) resultant force vector magnitudes, 3) coordination angle - the angle formed by the resultant vectors of each digit, 4) direction angles - the angle formed by each vector and the coordinate axes, and 5) center of pressure locations. It was shown that the resultant force magnitude of the index finger exceeded that of the thumb by 0.8 ± 0.3 N and that the coordination angle between the digit resultant force vectors was 160.2 ± 4.6°. The experimental apparatus and analysis methods provide a valuable tool for the quantitative examination of biomechanics and motor control during dexterous manipulation.
THE EFFECT OF PREGNANCY AND POSTPARTUM RECOVERY ON THE VISCOELASTIC BEHAVIOR OF THE RAT CERVIX
Barone WR, Feola AJ, Moalli PA and Abramowitch SD
The objective of this study was to elucidate the normal functional adaptations of the cervix in pregnancy. Utilizing a Long-Evans rodent model, the cervix was divided into distal and proximal portions for virgin, mid-pregnant, and four weeks postpartum animals. The quasi-linear viscoelastic theory describes the elastic and viscous behavior of the cervix. A hydroxyproline assay was used to measure collagen content. The nonlinearity of the elastic response significantly increased throughout the entire cervix during pregnancy when compared to virgin samples (p < 0.05) and was similar to virgin samples postpartum. All viscous behavior, except for the short-term relaxation of the proximal cervix, significantly differed for pregnant specimens (p < 0.05) and remained similar to pregnant samples postpartum. Collagen content was found to increase by mid-pregnancy only in the proximal cervix when compared to virgin. Distal and proximal portions, however, were found to differ in collagen content at all time points (p < 0.05). This study finds that the cervix becomes elastically stiffer with increasing strain and exhibits increased viscous behavior during pregnancy, with incomplete recovery postpartum. These alterations allow for quick dissipation of loads, and are likely related to altered matrix organization and porosity reported by others.
BENEFICIAL EFFECTS OF EXOGENOUS CROSSLINKING AGENTS ON SELF-ASSEMBLED TISSUE ENGINEERED CARTILAGE CONSTRUCT BIOMECHANICAL PROPERTIES
Elder BD, Mohan A and Athanasiou KA
As articular cartilage is unable to repair itself, there is a tremendous clinical need for a tissue engineered replacement tissue. Current tissue engineering efforts using the self-assembly process have demonstrated promising results, but the biomechanical properties remain at roughly 50% of native tissue.
Microfluidic Droplet Consistency Monitoring and Cell Detection via Laser Excitation
Tkaczyk AH, Tkaczyk ER, Norris TB and Takayama S
Microfluidic droplets formed in emulsions are used in a variety of analytical techniques and hold great potential for future scientific and commercial applications. Our experiments merge quantitative quality engineering methods into the microdroplet field. We present a unique microdroplet generation and consistency monitoring system with laser optics excitation and detection. Our setup analyzes each droplet with sub-millisecond signal resolution and single photon accuracy, and is compatible with process control methods. To demonstrate the consistency of microdroplet generation over time, we measure and examine the mean frequency of aqueous plug-shaped droplet (microplug) formation in oil phase, as well as the mean length of plugs, and the interval between consecutive droplets. We also demonstrate the detection of cancer cells encapsulated within aqueous microdroplets in continuous oil phase flow. Two-channel optical monitoring allows for the simultaneous and independent inspection of both microdroplet generation and identification of green fluorescent protein-labelled cancer cells within the droplets. Increased accuracy and consistency are central to many established and developing microfluidic technologies. A systematic, quantitative approach as demonstrated with our experiments may be essential in the development of advanced microfluidic concepts that require exacting reproducibility and would greatly benefit from incorporated automated measurement techniques for process control.
FLOW DYNAMIC COMPARISON BETWEEN RECESSED HINGE AND OPEN PIVOT BI-LEAFLET HEART VALVE DESIGNS
Govindarajan V, Udaykumar HS and Chandran KB
The flow dynamics through the peripheral and hinge regions of a bi-leaflet mechanical heart valve are complex and result in abnormally high shear stresses particularly during the closing phase of the valve function. It has been observed that, the late stages of closure is more significant in the dynamics of platelet activation; therefore, the later stages of closure is simulated by solving the two-dimensional Navier-Stokes equations using an Eulerian Levelset based sharp interface Cartesian grid method. Using a fixed Cartesian mesh incorporating local mesh refinement for solution accuracy and efficiency, the flow through and within a recessed hinge design and an open pivot hinge design is compared. Platelets are modelled as point particles by Lagrangian particle tracking algorithm with one way coupling. A dilute particle flow is assumed and particle-particle interactions are neglected. It was observed that the hinge region of the open pivot valve indicated a lower potential for activation of platelets compared to that in valves with a recessed hinge design.
VASCULAR MECHANICS, MECHANOBIOLOGY, AND REMODELING
Humphrey JD
Arteries exhibit a remarkable ability to adapt in response to sustained alterations in hemodynamic loading as well as in response to disease, injury, and clinical treatment. A better understanding of such adaptations will be aided greatly by formulating, testing, and refining appropriate theoretical frameworks for modeling the biomechanics and associated mechanobiology. The goal of this brief review is to highlight some recent developments in the use of a constrained mixture theory of arterial growth and remodeling, with particular attention to the requisite constitutive relations, and to highlight future directions of needed research.
ELECTROMAGNETICALLY INDUCED DISTORTION OF A FIBRIN MATRIX WITH EMBEDDED MICROPARTICLES
Scogin T, Yesudasan S, Walker MLR and Averett RD
Blood clots occur in the human body when they are required to prevent bleeding. In pathological states such as diabetes and sickle cell disease, blood clots can also form undesirably due to hypercoagulable plasma conditions. With the continued effort in developing fibrin therapies for potential life-saving solutions, more mechanical modeling is needed to understand the properties of fibrin structures with inclusions. In this study, a fibrin matrix embedded with magnetic micro particles (MMPs) was subjected to a magnetic field to determine the magnitude of the required force to create plastic deformation within the fibrin clot. Using finite element (FE) analysis, we estimated the magnetic force from an electromagnet at a sample space located approximately 3 cm away from the coil center. This electromagnetic force coupled with gravity was applied on a fibrin mechanical system with MMPs to calculate the stresses and displacements. Using appropriate coil parameters, it was determined that application of a magnetic field of 730 A/m on the fibrin surface was necessary to achieve an electromagnetic force of 36 nN (to engender plastic deformation).