Originating in South America, wheat blast disease has spread to both Asia and Africa and is considered a significant threat to food security. Bangladesh experienced the first outbreak of wheat blast outside of the Americas in 2016. Shortly thereafter, the blast-resistant variety BARI Gom 33 was released. Seeds of this variety are however not as widely available as required, although the disease threat remains. While varietal mixtures have been shown to mitigate some symptoms and yield losses associated with other fungal diseases in wheat, there is a complete research gap on this topic as it pertains to wheat blast. As such, we evaluated the potential of using BARI Gom 33 as a component of a variety mixture under high disease pressure in Bangladesh. During three cropping seasons, blast symptoms and yield were determined in a field experiment for the highly blast-susceptible variety BARI Gom 26, the moderately susceptible BARI Gom 30, the resistant BARI Gom 33, and seven mixture combinations of the three varieties using artificial inoculation to increase disease pressure. In addition to wheat blast, leaf blight (BpLB) symptoms were observed and evaluated. While yields of the susceptible varieties were severely affected by blast even after fungicide application, disease-inflicted yield loss without fungicide was only 15% for sole BARI Gom 33 and did not differ significantly from yield losses in BARI Gom 33 and BARI Gom 30 mixtures. Furthermore, in the mixture containing 67% BARI Gom 33 and 33% BARI Gom 30, blast incidence and severity were reduced by 25% and 16%, respectively, in comparison to weighted values in sole stands. Conversely, mixing varieties tended to increase the symptoms of BpLB. Under high wheat blast pressure, fungicide protection against blast was relatively weak, underscoring the importance of resistant varieties. Although variety mixtures did not increase yield, the yield advantage of BARI Gom 33 was maintained when its seeds were mixed with the less resistant BARI Gom 30. This study confirms recommendations that farmers should use BARI Gom 33 as a first line of defense against wheat blast in Bangladesh. Yet where farmers cannot access sufficient BARI Gom 33 seed for planting, our data suggest that agricultural extension services can recommend this variety with non-resistant cultivars as interim strategy without significant risk of yield loss.

This study evaluated effects to native plant and weedy Brassicaceae species growing in areas potentially affected by drift of glyphosate used with glyphosate-resistant canola (). Ten native grass and forb species were selected based on importance in prairie areas of North Dakota, US; and four introduced Brassicaceae species (, , , and ssp. ) were selected based on their ability to cross with . Greenhouse-grown seedlings were treated with 0 (carrier control), 0.00056, 0.0032, 0.018 and 0.1 × a field application rate (FAR) of 829 g ha acid glyphosate (g acid equivalent or a.e. ha), along with no spray plants; with each treatment repeated in two experiments. Shoot dry weight and height were measured 14 days after treatment, and data were subjected to analysis of variance or covariance followed by a Dunnett's multiple comparison test to obtain No-Observed-Adverse-Effect-Rates (NOAERs) for both parameters. A Weibull regression was used to obtain the rate producing a 25% reduction (ER25) for shoot dry weight or height for a limited number of species and experiment combinations. Based on NOAER values for both shoot dry weight and height, most native species had reductions in growth with 0.1 x FAR resulting in NOAERs of 0.018 × FAR for at least one experiment. was the most sensitive native species, with a NOAER of 0.0032 x FAR for shoot dry weight and one experiment for height. The Brassicaceae species responded similarly to glyphosate as the native species, with NOAER values ranging from 0.0032 to 0.018 × FAR. Only four species had valid regression analyses for shoot dry weight or height resulting in ER25 values between 0.007 and 0.054 x FAR. and were not affected by glyphosate as indicated by NOAER values. This study indicated that drift concentrations between approximately 0.003, but more commonly ≥ 0.1 × FAR (2.49 and 82.9 g ha acid glyphosate, respectively) may affect the growth and potential competitiveness of selected native plant species, and Brassicaceae species sexually compatible with glyphosate-resistant in North Dakota.

Cassava plays a key role in the food security and economy of Cameroon, but its production is constrained by cassava mosaic disease (CMD). However, comprehensive surveys of CMD in Cameroon have been lacking. This study aimed at evaluating the current status of CMD and its whitefly vector. Field surveys were conducted in 2020 using a sampling, diagnostics and data storage protocol that has been harmonized across 10 West and Central African countries for ease of comparison. Thirty plants per field were assessed for CMD severity, whitefly abundance and source of infection. Surveys were conducted in 343 fields and confirmed the presence of CMD in all 10 regions of Cameroon. Among the 10,057 assessed plants, 33.07% were deemed healthy (asymptomatic). At the field level, only 6.7% fields were found to be healthy. The mean CMD incidence across the country was 66.93%, and the mean severity score was 2.28. The main mode of infection was likely through contaminated cuttings. The mean whitefly count per plant was 5.78. This study is the first countrywide survey of CMD in Cameroon and provides insights that can be useful for improving the country's CMD intervention and management strategies.

Cassava mosaic disease (CMD) is the main threat to cassava ( Crantz) production in Benin. This study was conducted to assess CMD incidence, disease severity, and adult whitefly () populations in 11 regions of Benin. A total of 180 cassava fields across the country were assessed during June-December 2020 following the harmonized protocol of the Central and West African Virus Epidemiology program. Based on symptoms observation, CMD was present in all surveyed fields in Ouémé and Alibori regions. The highest disease incidence levels were observed in Malanville (100%), Kpomassè (86.67%), Kandi and Zagnanado (both 81.67%), Ségbanan (80%), and Avrankou (76.67%) districts. The highest mean severity scores were in Couffo (3.68), Mono (3.63), and Atlantique (3.33) regions, while the lowest was in Alibori (2.37). Adult whitefly populations (mean number/plant) were highest in Couffo (15.88) and Mono (13.00) regions and lowest in Donga (0.06). Significant relationships were found between CMD severity and whitefly abundance () but there was no significant relationship between whitefly numbers and CMD incidence (). These findings indicate that CMD has expanded its range across Benin. They also provide a basis for designing a response strategy for the control of cassava virus diseases such as CMD.

Stem borers are major insect pests of maize in Uganda. A study was conducted in 2014-2016 to assess the performance of Bt hybrids expressing Cry1Ab (event MON810) against the two major stem borer species in Uganda - the African stem borer () and the spotted stem borer () - under artificial infestation. The study comprised 14 non-commercialized hybrids, including seven pairs of Bt and non-Bt hybrids (isolines), three non-Bt commercial hybrids and a conventional stem borer resistant check. All stem borer damage parameters (leaf damage, number of internodes tunneled and tunnel length) were generally significantly lower in Bt hybrids than in their isolines, the conventionally resistant hybrid, and local commercial hybrids. Mean yields were significantly higher by 29.4-80.5% in the Bt hybrids than in the other three categories of non-Bt hybrids. This study demonstrated that Bt maize expressing Cry1Ab protects against leaf damage and can limit entry of stem borers into the stems of maize plants, resulting in higher yield than in the non-transgenic hybrids. Thus, Bt maize has potential to contribute to the overall management package of stem borers in Uganda.

Weed competition is the major biological stress affecting cassava production in smallholder farms in West and Central Africa, where yields are low compared with those in Asia and Latin America. Options for improved weed management are crucial in increasing productivity. Selected pre- and post-emergence herbicides, integrated with appropriate tillage and plant spacing, were tested in 96 sites in four locations in Nigeria, 24 in 2016 and 72 in 2017. Trials were split plots with six pre-emergence herbicides and no post-emergence treatment as main plots. Subplot treatments were four post-emergence herbicides, weeding with a motorized rotary weeder, short- and long-handled hoes, and no post-emergence weed control, i.e., regardless of pre-emergence treatments. Indaziflam-based treatments, irrespective of post-emergence treatment, and flumioxazin + pyroxasulfone applied pre-emergence followed by one weeding with a long-handled hoe provided >80% control of major broadleaf and grass weeds. Compared with herbicide use, farmer control practices (53%) were not efficient in controlling weeds. The highest root yield was produced where (1) s-metolachlor was combined with atrazine, and one weeding with a long-handled hoe or clethodim with lactofen, and (2) indaziflam + isoxaflutole was combined with glyphosate. An increase in root yield from 3.41 to 14.2 t ha- and from 3.0 to 11.99 t ha- was obtained where herbicides were used compared with farmers' practice and manual hoe weeding. Our results showed that integrating good agronomic practices with safe and effective use of appropriate herbicides can result in root yield >20 t ha i.e., twice the national average root yield of 8-12 t ha, with >50% net profit. The use of appropriate herbicides can reduce the amount of manual labor required and improve livelihoods, specifically for women and children. Smallholder cassava farmers would require continuous training on the safe use and handling of herbicides to improve efficiency and prevent adverse effects on humans and the environment.

- Fusarium wilt, caused by f. sp. (Foc), is one of the most destructive diseases affecting banana crops worldwide. Therefore, the development of resistant cultivars is a promising alternative to mitigate the effects of the disease on banana plantations. The objectives of this study were to induce somaclonal variation in banana cultivars of the Silk and Cavendish types and to select somaclones resistant to subtropical race 4, thereby enabling the production of fruit in areas where this race is present. Shoot clump apexes of the Grand Naine and Maçã (Silk) cultivars were grown in MS medium. The cultures were subcultured four times. They were then challenged with fusaric acid (FA) in an experiment consisting of four treatments with different concentrations (0.1, 0.2, 0.3, and 0.4 mM) and five repetitions, each consisting of a Petri dish containing seven multiple shoot clumps in MS culture medium supplemented with 2.5 mg/L benzylamine purine. Multiple shoot clumps without the addition of FA were also used in the experiment, and were subcultured three times and maintained in a dark room. The multiple shoot clumps that survived the treatment with FA were transferred to MS medium and maintained in the growth chamber in the presence of light. The regenerated plants were later planted in tanks containing soil infested with an isolate classified as Foc subtropical race 4 (Foc STR4), and were evaluated for resistance to the pathogen at 90 days after inoculation (d.a.i.). Pathogen structures were confirmed by root clarification and root staining technique. All somaclones of the Maçã (Silk) cultivar were susceptible to Fusarium wilt and two somaclones of the Grand Naine cultivar were selected as resistant. The addition of FA as a selective agent was effective in the selection of somaclones among plants of the Grand Naine cultivar, as shown by the selection of two somaclones resistant to Foc STR4. The next step will consist of the agronomic and market potential validation of the selected somaclones, aiming to confirm their potential use by producers.

Farmers continue losing substantial quantities of grain during storage due to damages from pests including insects. Hermetic bags, being promoted in Ethiopia, could be viable alternatives to traditional methods and insecticides that are commonly used by farmers to store grain. However, the economics and determinants behind farmers' decisions to use different storage methods are poorly understood. This study sought to ascertain the economics of hermetic grain storage technology among 450 representative small-scale maize farmers in northwestern Ethiopia. Gross margin (GM), and the marginal rate of return (MRR) were employed to estimate the economic costs and benefits of storage methods, while a multivariate probit regression model was employed to analyze the determinants of farmers' decision to store maize with a given storage method. The results show that farmers used a combination of different storage techniques: 19.6% did not store grain, 87.8% used traditional methods with pesticide, and 66.7% used Purdue Improved Crop Storage (PICS) hermetic bags. Farmers who used hermetic bags also used other mentioned storage techniques. PICS had the highest GM (US$21.77 100 kg) and MRR (3.196), indicating that they were the most profitable. Moreover, a household could obtain an additional net cash flow of US$5.02 100 kg PICS bag per season after 9.6 months of storage. Farmers' decisions to use PICS bags were influenced by several factors including access to information, the initial cost, and storage capacity of the technology. Thus, increasing awareness and improving supply chain efficiency to reduce the cost of the PICS bags would improve adoption rates.

Yield losses from rice stem borers depend on the nature of the rice variety, the timing of attack, and the composition of the stem borer species assemblage. This study uses a range of phenotyping methods to distinguish different categories of herbivore-rice interaction that determine relative damage levels (dead heart and whitehead-panicles) and yield losses to varieties exposed to stem borers. Phenotyping studies were conducted in a greenhouse, screen house and field using two stem borer species ( [yellow stem borer - YSB] and [striped stem borer - SSB]) and 12 rice lines. Only YSB displayed oviposition preferences across rice varieties. Both stem borer species performed better (greater survival, shorter development times, heavier pupae) on rice at vegetative compared to reproductive growth stages, and SSB was less capable than YSB of developing on rice at reproductive growth stages. Stem borer larval survival, body weight, development time, and tiller damage across different rice varieties in greenhouse experiments was poorly correlated between the two stem borer species and for each of the species on rice at two different plant growth stages (vegetative and reproductive). In contrast, rice tillering and yield responses to the two stem borer species were often highly correlated, but only when plants were attacked at the reproductive stage. Short-term, controlled experiments revealed aspects of host resistance and relative changes in plant biomass and yield in response to damage (condition change). However, the results from controlled environments and field-plots were not generally correlated because plant vulnerability, i.e., relative exposure to stem borer attack due to crop duration, largely determined field damage. We recommend that phenotyping studies should differentiate between the resistance, tolerance and vulnerability of rice lines to complexes of stem borers in a given region. Single stem borer species experiments under controlled environments are useful to gain knowledge of the nature of rice-stem borer interactions; however, field testing with comparative treatments, particularly under high soil fertilizer levels that increase plant attractiveness, are better for assessing the relative propensities of rice varieties to incur damage and yield losses due to complexes of stem borers.

Smallholder maize farmers in Africa experience pre- and post-harvest production stresses either individually or in combination at different stages of the crop cycle. The maize weevil is among the major post-harvest storage pests. A strategy to address this problem is to develop and promote high yielding maize germplasm with resistance to multiple stresses. A study was conducted to: 1) assess yield and agronomic performance of testcross hybrids developed from early generation lines; and 2) assess the response of the testcross hybrids to infestation with Fifty-eight drought-tolerant testcross hybrids were evaluated for agronomic performance and weevil resistance at four environments in Uganda in 2016. Hybrid G39 (L2/T2) had the best grain yield performance; it significantly out-performed the best check by 11.4% in all environments. Hybrid grain from field trials was subjected to infestation in a choice and no choice test under laboratory conditions. Hybrids G56 (L49/T2) and G58 (L51/T2) had the least weevil damage and were rated as resistant to . The numbers of damaged kernels, number of exit holes and ear aspect were positively correlated with the grain weight loss. The results suggest possibilities for simultaneous selection for high grain yield and storage insect pest resistance among drought-tolerant genotypes. Use of high-yielding and resistant maize hybrids to storage insect pest should be promoted for increased maize production and managing post-harvest losses due to the maize weevil in smallholder farming communities in Africa.

Northern corn leaf blight (NCLB) incited by the fungus is a foliar disease that significantly limits maize production and productivity in West and Central Africa (WCA), particularly in the mid-altitudes but during the last decade it has become a menace in lowland agro-ecologies. The most economical and environmentally friendly disease management strategy is the cultivation of maize varieties resistant or tolerant to NCLB. However, no early maturing (EM) and extra-early maturing (EEM) NCLB resistant varieties are commercially available in WCA. One hundred inbred lines each of EM and EEM derived from tropical maize germplasm were inoculated with a virulent isolate of at five locations in Nigeria during the 2017 and 2018 growing seasons. The objective of the study was to identify promising NCLB resistant lines and to investigate inter-relationships among the traits. Analysis of variance revealed highly significant genotype and genotype by environment (G × E) interactions for disease severity, grain yield (GYLD), and other agronomic traits. The average disease severity (TURC) values ranged from 1.9 to 5.8 and 2.9 to 5.7 for the EM and EEM inbred lines, respectively. The levels of reaction of the inbred lines to NCLB ranged from highly resistant to highly susceptible. Stepwise regression analysis showed that ears per plant, ear and plant aspects were significantly influenced by the disease scores. Ears per plant, ear and plant aspects, TURC and GYLD traits were employed to develop a base index (BI) for selecting NCLB resistant inbred lines for hybrid development. TZEI 135 and TZEEI 1 were outstanding in GYLD and also had the highest positive BI values in the EM and EEM inbred lines, respectively. The identification of NCLB resistant lines in this study has set the premise for development of NCLB resistant hybrids for WCA as well as the improvement of tropical maize breeding populations for NCLB resistance.

The cabbage stem flea beetle, L. is a major pest of winter oilseed rape in several European countries. Traditionally, neonicotinoid and pyrethroid insecticides have been widely used for control of , but in recent years, following the withdrawal of neonicotinoid insecticide seed treatments, control failures have occurred due to an over reliance on pyrethroids. In line with previous surveys, UK populations of were found to exhibit high levels of resistance to the pyrethroid lambda-cyhalothrin. This resistance was suppressed by pre-treatment with the cytochrome P450 inhibitor PBO under laboratory conditions, suggesting that the resistance has a strong metabolic component. The L1014F (kdr) mutation in the voltage-gated sodium channel, which confers relatively low levels (10-20 fold) of resistance to pyrethroids, was also found to be widespread across the UK regions sampled, whereas the L925I (s-kdr) mutation was much less common. The current survey also suggests that higher levels of pyrethroid resistance have spread to the North and West of England, and that resistance levels continue to remain high in the South East.

In South Asia's rice-based cropping systems, most farmers flood and repetitively till their fields before transplanting. This establishment method, commonly termed puddled transplanted rice (TPR), is costly. In addition, it is labor and energy intensive. To increase labor and energy efficiency in rice production, reduced or zero-tilled direct seeded rice (ZT-DSR) is commonly proposed as an alternative tillage and crop establishment (TCE) option. Effective management of weeds in ZT-DSR however remains a major challenge. We conducted a four-year experiment under a rice-maize rotation in Northwestern Bangladesh in the eastern Gangetic Plains to examine the performance of two TCE methods and three weed management regimes (WMR) on the diversity and competitiveness of weed communities in the rice phase of the rotation. The Shannon-Weiner Diversity Index, a measure of species diversity, was significantly greater under ZT-DSR than puddled TPR. It was also greater under no weed control (Weedy) and two manual weeding (MW) treatments compared to chemical herbicide with manual weeding (C + MW). In DSR Weedy plots, weed communities began shifting from grasses to sedges from the rotation's second year, while in the ZT-DSR and C + MW treatments, sedges were consistently predominant. In both puddled TPR Weedy and TPR C + MW treatments, broadleaves and grasses were dominant in the initial year, while sedges dominated in the final year. There were significant main effects of year (Y) and weed management regime (WMR), but not of TCE. Significant Y × TCE and TCE × WMR interaction effects on rice yield were also observed. Grain yields under ZT-DSR were similar to puddled TPR. ZT-DSR with one application of pre-emergence herbicide followed by one hand weeding at 28 days after establishment however resulted in significantly higher grain yield (5.34 t ha) compared the other weed management regimes. Future research should address methods to effectively manage weed community composition shifts in both ZT-DSR and TPR under rice-maize rotations utilizing integrated and low-cost strategies that can be readily applied by farmers in the eastern Gangetic Plains.

Trade and transport of goods is widely accepted as a primary pathway for the introduction and dispersal of invasive species. However, understanding commodity flows remains a challenge owing to its complex nature, unavailability of quality data, and lack of systematic modeling methods. A robust network-based approach is proposed to model seasonal flow of agricultural produce and examine its role in pest spread. It is applied to study the spread of , a devastating pest of tomato in Nepal. Further, the long-term establishment potential of the pest and its economic impact on the country are assessed. Our analysis indicates that regional trade plays an important role in the spread of . The economic impact of this invasion could range from USD 17-25 million. The proposed approach is generic and particularly suited for data-poor scenarios.

Host plant resistance has received considerable attention for the management of insect herbivores on crop plants. However, resistance is threatened by the rapid adaptation of target herbivores towards virulence (the ability to survive, develop and damage a host with major resistance genes). This study examines the potential costs and benefits of adaptation for virulence in herbivores. We continuously reared planthoppers, , on two susceptible and three resistant rice, , varieties for 20 + generations. We then assessed the performance of selected planthoppers across a range of rice lines with distinct resistance genes. We found that planthoppers with long-term exposure to resistant hosts (particularly IR62 with the and gene loci, and PTB33 with the , and gene loci) gained virulence against related varieties with the same and different resistance genes, but planthoppers adapted to the resistant host IR65482-4-136-2-2 ( locus) had reduced performance on phylogenetically distant plants with distinct resistant genes. In choice bioassays, avirulent planthoppers showed marked preferences for susceptible lines, whereas virulent planthoppers were less selective of varieties. We also examined whether virulence was associated with insecticide susceptibility. We tested susceptibility to three insecticides using a topical application method. Populations selectively reared on IR65482-4-136-2-2 had increased susceptibility to imidacloprid and fipronil, representing a possible trade-off with virulence. In contrast, a population with virulence to the highly resistant variety PTB33 was 4.88 × more resistant to imidacloprid and 3.18 × more resistant to BPMC compared to planthoppers of the same origin but reared on the susceptible variety IR22. Our results suggest complex relations between insecticide resistance and virulence that vary according to insecticidal toxins and resistance genes, and include potentially increased insecticide-susceptibility (a trade-off) as well as common detoxification mechanisms (a benefit).

The first occurrence of wheat blast in 2016 threatened Bangladesh's already precarious food security situation. The Bangladesh Agricultural Research Institute (BARI), together with the International Maize and Wheat Improvement Center (CIMMYT) developed and released the wheat variety 33 that is resistant to wheat blast and other common diseases. The new variety provides a 5-8% yield gain over the available popular varieties, as well as being zinc enriched. This study examines the potential economic benefits of in Bangladesh. First, applying a climate analogue model, this study identified that more than 55% of the total wheat-growing area in Bangladesh (across 45 districts) is vulnerable to wheat blast. Second, applying an impact assessment framework, this study shows that with an assumed cumulative adoption starting from 2019-20 and increasing to 30% by 2027-28, the potential economic benefits of the newly developed wheat variety far exceeds its dissemination cost by 2029-30. Even if dissemination of the new wheat variety is limited to only the ten currently blast-affected districts, the yearly average net benefits could amount to USD 0.23-1.6 million. Based on the findings, international funder agencies are urged to support the national system in scaling out the new wheat variety and wheat research in general to ensure overall food security in Bangladesh and South Asia.