This study aimed to measure the psychometrics qualities of an extended model of the Theory of Planned Behavior (TPB) applied to plasma donation, and its relevance in the evaluation of interventions aiming at converting whole-blood donors (WBD) to plasma donation.

T lymphocyte collection is essential for CAR T-cell engineering in refractory hematologic malignancies but needs to be optimised. No guidelines have been established for predicting the amount of T lymphocytes to be collected. The quantity of lymphocytes and especially T cells collected depends on the pre-cytapheresis lymphocyte blood level (pcLBL) and the number of blood volumes (BVs) processed. Our aim was to define and standardise a simple method for predicting the number of T lymphocytes collected, taking into account the number of BVs processed and the pcLBL regardless of the procedures defined by different companies.

Neonatal hyperbilirubinemia, or newborn jaundice, is a common condition caused by high bilirubin levels. Blood group incompatibility between mother and baby is a major cause. This study examined the link between different blood group incompatibilities and their management in newborns with jaundice.

Therapeutic plasma exchange (TPE) is the most widely used artificial liver support system in liver failure patients.

Megaloblastic anemia (MA) is a rare pathology in childhood due, in the majority of cases, to a deficiency of folic acid and/or vitamin B12 (cobalamin). This study aims to determine the epidemiological, clinical, and paraclinical profiles of MA in children and to specify its etiologies, therapeutic modalities, and treatment responses.

In the third week of November 2024, a critical incident involving the refusal of a blood transfusion was reported at our hospital. The case involved a 65-year-old Indian patient who had been admitted for a proposed stoma closure surgery. Although the healthcare team deemed an urgent blood transfusion necessary as part of the patient's treatment plan, the transfusion was refused due to misinformation from the patient's attendants regarding the patient's original blood type. Their refusal was also driven by a fear of the potential consequences of an erroneous mismatched blood transfusion. The blood transfusion centre (BTC) laboratory confirmed the patient's blood type as B Rh (D) positive. However, the attendants raised concerns about the accuracy of this blood grouping, citing previous misunderstandings and misinformation that led them to believe the patient was AB Rh (D) positive until that point. Despite receiving multiple assurances and thorough explanations from the attending physician and nursing staff, the attendants remained distrustful of the BTC laboratory results and requested a re-evaluation of the patient's blood type. As a result, a fresh blood sample was collected for repeat typing. After a one-on-one discussion with our transfusion medicine specialist, the attendants were ultimately convinced of the confirmed blood type. Subsequently, three compatible packs of packed red blood cells (PRBC) of B Rh (D) positive were issued to the patient over the next three consecutive days from our blood centre. This situation underscores the importance of effective communication and education regarding the patient's actual blood type. Our report further details the incident, its consequences, the associated ethical dilemmas, and recommendations to prevent similar occurrences in future.

Pediatric patients with sepsis are frequently subjected to red blood cell (RBC) transfusions but yet its association with mortality is still controversial.

Plasma-derived medicines (PDMs) are essential for treating various disorders and require large volumes of human plasma. The debate on voluntary and compensated plasma donation continues, while WHO advocating for voluntary donations. This study examines factors influencing plasma donation, focusing on the effectiveness of voluntary donation and identifying key motivators and barriers.

Guillain-Barre syndrome (GBS) is a disease entity described in literature since 1859. It is associated with various etiological, clinical and immunological factors with prognostic predictive value. Both Intravenous immunoglobulin (IVIG) and Therapeutic Plasma Exchange (TPE) have been regarded as the first-line treatment for GBS. Certain diagnostic tools help us in early identification of GBS subtypes that may aid clinical management. Here, we have discussed six paediatric cases of GBS of Acute Motor Axonal Neuropathy (AMAN) subtype that were considered for TPE. 5 out of 6 patients were eventually weaned from mechanical ventilation and discharged from the hospital. This study emphasizes the role of TPE in management of severe IVIG refractory GBS with axonal involvement that can be beneficial to the patient. TPE may be considered early in GBS cases with axonal involvement.

Plateletpheresis (PP) has become increasingly prevalent due to its cost-effectiveness and fewer immunological and infectious complications for recipients. This study compares hematological indices of platelet donors and instrument-related parameters in high-yield PP donors using Haemonetics MCS+ and Trima Accel.

Platelet storage duration may influence transfusion effectiveness and patient outcomes.The present study aimed to evaluate the effect of platelet storage duration on platelet increment and clinical outcomes in patients admitted to the intensive care unit (ICU).

Blood donor cohorts are an underappreciated resource for surveillance and public health programming for infectious diseases. The incidence of hepatitis C virus (HCV) infection was evaluated in repeat blood donors in Georgia.

The electronic prescription of Labile Blood Products was recommended in a 1997 directive, to ensure transfusion safety in healthcare services. Following multiple institutional reminders by the Haemovigilance Unit of the Marseille Public Hospitals (APHM), the board of directors decided to set up a project for electronic transfusion records. Here we present the issues arising from this computerisation and the impact of this change of practice on the APHM and the Etablissement Français du Sang PACA-Corse (EFS PACA-Corsica; French Blood Institute Provence-Alpes-Côte d'Azur-Corsica). Concerning the APHM, we were overly optimistic when designing the prescription of Labile Blood Products (LBP), thinking that prescribers would choose the correct product qualifications. Unfortunately, the choice of LBP qualifications was excessive, resulting in product reception being blocked for the medical units. Furthermore, we were also faced with a resistance to change from the older doctors, while the younger doctors and junior doctors at ease with computers rapidly adhered to the tool. Concerning the EFS PACA-Corsica, this change in practice disconnected the link between the software in charge of red cell immunohaematology and LBP delivery, requiring the development of a new tool to re-establish a link. The EFS demanded a remote print-out, as a back-up for the electronic delivery of the prescription. The issue around identity discordance reinforced the ties between the APHM Haemovigilance and Identity Vigilance Units and the EFS. The partnership between our two structures was beneficial for the implementation of electronic transfusion records. The APHM gained in safety, obliging prescribers to note the duration of transfusion, and in healthcare traceability (reception, transfusion, adverse event reporting etc.). For the EFS PACA-Corsica, despite the difficulties encountered with software when the tool was first implemented, electronic nominative prescription made tasks simpler, gave better access to transfusion data, and reduced the number of phone calls from medical units inquiring about patient immunohaematology results. The comprehension and attentiveness between our two entities enabled us to complete this project and to resolve the problems as they appeared.

In this study, we aimed to investigate the current genetic diversity and provide additional insights into the origins of the Algerian population by analyzing the frequencies of HLA -A,-B,-DRB1,-DQB1 alleles and associated haplotypes. We analyzed 1,082 unrelated healthy Algerian individuals, who were potential kidney donors, recruited and assessed in the Immunology Department of CHU Mustapha in Algiers over a 10-year period (2009-2019). HLA genotyping was performed by Polymerase Chain Reaction Sequence Specific Primers (PCR-SSP). The allelic typing estimate and haplotype frequencies were calculated using the R studio® software and Haplotype frequency estimation from the website https://hla-net.eu/. We identified a total of 20 HLA-A alleles, 31 HLA-B alleles, 13 HLA-DRB1 alleles and5 HLA-DQB1 alleles. Regarding the allele frequency, the haplotype frequency and the distribution with other population database, we were able to provide a description of Algerian genetic background and draw some anthropogenetic conclusions. This study of HLA polymorphism in the Algerian population shows a strong genetic similarity with the populations of southern Europe and North Africa (Morocco, Tunisia and Libya) with which it has shared a common history since antiquity. In addition to its contribution to anthropogenetics, our study, serve as a reference database for studies exploring associations between the HLA system and certain pathologies.

During the SARS-CoV-2 pandemic, there has been significant increased use of vvECMO as rescue therapy. Patients with COVID-19 as anticoagulation is needed for vvECMO support, may develop bleeding complications requiring an increased number of RBC transfusions. We would like to report the RBC transfusion needs following the implementation of an ECMO program. Data on blood usage in this population is important in view of the decline in donations due to the pandemic.

Immunized platelet transfusion refractoriness (IPTR) has a high incidence of death and complications. HLA-matched platelet unit can effectively treat HLA-immunized PTR. A cohort study was undertaken on nine patients with IPTR hematological disorders who underwent HLA-matched PLT units due to HLA immunization at the First Affiliated Hospital of Soochow University between April 2022 and April 2023. We calculated the 14-hour corrected count increments (14 h-CCI) to evaluate the effect of PLT transfusions. A 14 h- CCI > 5000 was considered to be a successful transfusion. A total of 113 PLT units were transfused to the nine patients with HLA-immunized PTR. Of the 113 PLT units, 50 were random, 34 were cross-matched, and 29 were HLA-matched. The median 14 h-CCI values were 1683 for random PLT units, 5246 for cross-matched PLT units, and 5643 for HLA-matched PLT units (P = 0.02). Moreover, 10, 25, and 43.8% of transfusions were successful for random, cross-matched, and HLA-matched PLT units with non-immune factors (P = 0.013). Regarding non-immune factors, we confirmed that random PLT units, infection, splenomegaly, and bleeding affected platelet transfusion increments (P < 0.05).

The main objective of these recommendations for good practices is to supplement the regulatory provisions of the decree of May 15, 2018 which sets the conditions for performing erythrocyte immunohematology tests in medical biology, in order to clarify their application for medical laboratories which conduct first-line immunohematology tests: erythrocyte phenotyping and detection of anti-erythrocyte antibodies. These recommendations cover all phases of a biological examination (pre-analytical, analytical, and post-analytical) as defined in the Public Health Code (Article L6211-2). Four levels of recommendations have been established: ● Recommended practices: complying with the requirements of the NF EN ISO 15189:2022 standard and ensuring optimal medical service delivery. ● Acceptable practices: situations which deviate from the best practices in immunohematology but still ensuring satisfactory patient management as well as transfusion and obstetrical safety. ● Non-recommended practices: not complying with the requirements of the NF EN ISO 15189:2022 standard and not allowing satisfactory patient management, but ensuring transfusion and obstetrical safety. ● Unacceptable practices: presenting a risk for transfusion or obstetrical complications. Compliance with these recommendations optimizes patient medical care, particularly in transfusion or obstetrical contexts.

Packed red blood cell (PRBC) transfusion is critical in managing pediatric patients with conditions requiring frequent transfusions, such as leukemia, thalassemia, and bone marrow disorders. Alloimmunization, the formation of antibodies against foreign antigens present in the donor's blood, is a significant complication of repeated transfusions. Further, auto/alloimmunization is influenced by multiple factors, including antigenic differences between donor and recipient and the recipient's immune status.