The objective of this study was to evaluate the effects of replacing raw rapeseed cake (RC) with fermented rapeseed cake (FRC) in the diet of dairy cows on methane (CH) production, ruminal fermentation, and milk production, composition, and fatty acid composition. The Hohenheim gas test (exp. 1) was initially used to evaluate RC and FRC as substrates. Following batch fermentation, an in vitro study (exp. 2) was performed to assess the effects of replacing RC with FRC at 28.75, 57.5, 86.25, and 115 g/kg (FRC25, FRC50, FRC75, and FRC100) in the total mixed rations (TMR). Based on the in vitro results, the control TMR (115 g/kg dry matter (DM) of RC; CONRC) and experimental TMR (115 g/kg DM of FRC; FRC100) were chosen for an in vivo assessment. In exp. 3, four ruminally cannulated cows were used in a replicated 2 (group) × 2 (period) crossover design and fed the TMR ad libitum. In exp. 4, twenty multiparous Polish Holstein-Friesian cows in their mid-lactation (148 ± 26 d in milk) were used in a completely randomized design. The cows were fed a partial mixed ration without the RC and FRC, and the RC and FRC were supplied in a concentrate feeder at 2.65 kg/d/cow. The FRC100 markedly decreased CH production by 12% and archaeal population without adversely affecting nutrient digestibility. The molar proportion of propionate was increased, and the molar proportion of acetate and butyrate and acetate to propionate ratio were decreased by FRC100. No significant effects on milk production or composition, except an increase in milk urea concentration, were observed in cows fed FRC100. Milk C18:2 -9, -11 concentration was greater, and n-6 to n-3 fatty acid ratio was lower for FRC100 than CONRC. ruminal degradation of RC and FRC were explored using techniques (exp. 5). The potential degradation and effective degradability of the DM, organic matter, and crude protein were significantly higher for FRC than RC. These results suggested that FRC could mitigate enteric CH production by decreasing archaeal abundances without adversely affecting milk production and ruminal fermentation in lactating cows.

The impact of plant-based diets on the digestive physiology of rohu fingerlings (10.66 ± 0.53 g) was evaluated. A diet with all protein supplied by fishmeal was included as a control (F). Four test diets containing 300 g/kg protein were formulated using the following plant ingredients and fishmeal in a 1:1 blend: almond oil-cake (FTC), duckweed (FLM), water fern (FSM) and combination of these three ingredients (FTCLMSM). The final body weight and specific growth rate were significantly higher in rohu fed diet FLM compared to the other treatments. Significantly lower feed conversion ratio in rohu fed diet FLM showed that diet was utilized efficiently in this feeding regime compared to the other diets. The composition of diets also influenced the digestive enzyme activities of the fish. Thus, amylase, trypsin and chymotrypsin activities were significantly higher in rohu fed diet FLM compared to the rohu fed the other diets. Protease activity was significantly higher in rohu fed diets FTC and F and lipase activity was significantly higher in rohu fed diet FTC compared to the rohu fed the other diets. The inclusion of raw duckweed in feed replaced 300 g/kg of dietary fishmeal without affecting growth.

Feed scarcity is a major challenge for livestock production in West Africa, especially during the dry season when grass quality and quantity on grazing lands are inadequate. In the dry season, crop residues are a key source of livestock feed. The residues of grain legumes, also known as grain legume fodders (GLFs), are stored and traded for feeding in the dry season. The objectives of our experiment were to evaluate the effects of storage conditions and duration on dry matter (DM) and nutritional quality of GLFs, and to assess the risk of aflatoxin in stored groundnut fodder. The experiment was designed as a factorial trial with 18 treatment combinations with four replicates (4 farms). The treatments included: 3 types of GLFs (cowpea, groundnut and soybean fodder), 3 types of storage locations (rooftop, room and tree-fork) and 2 types of packaging (packed in polythene sacks and unpacked but tied with rope). Over a 120 day storage period, DM quantity reduced by an average of 24 % across all storage conditions, showing a range from 14 % in the best condition (sacks and rooms) to 35 % in the worst condition (bundles tied with rope and stored on rooftops or tree-forks). Soybean fodder had no leaves, the lowest crude protein content (CP) and organic matter digestibility (OMD), and the highest content of cell wall components compared to cowpea and groundnut fodder. These nutritional quality parameters in soybean fodder hardly changed during storage. Cowpea and groundnut fodder showed a decrease in leaf-to-stem ratio (LSR), CP and OMD, and an increase in the content of cell wall components during storage, but their nutritional value remained better than that of soybean fodder. Storage in sacks resulted in less DM loss, in less reduction of LSR and in a smaller increase of the content of cell wall components than storage of bundles tied with rope. Our study shows that the DM loss, the decrease in LSR, and the increase in the content of cell wall components can be prevented partly by storing GLFs in sacks instead of tying bundles with rope, and to a minor extent by storing in rooms instead of in the open air. Aflatoxin was not detectable in the groundnut fodder samples. Our results highlight that attention to storage conditions can improve the feeding value of GLFs which are key for livestock nutrition during the dry season.

Nutritional intervention in older dogs aims to increase lifespan and improve life quality as well as delay the development of diseases related to ageing. It is believed that active fractions of mannoproteins (AFMs) obtained through extraction and fractionation of yeast cell walls () may beneficially modulate the immune system. However, studies that have evaluated this component and the effects of ageing on the immune system of dogs are scarce. This study aimed to evaluate the immunological effects of AFMs in adult and elderly dogs. Three extruded iso-nutrient experimental diets were formulated: without addition of AFM (T0); with AFM at 400 mg/kg (T400); and with AFM at 800 mg/kg (T800). Thirty-six beagle dogs were used, and six experimental treatments, resulting in combinations of age (adult and elderly) and diet (T0, T400, and T800), were evaluated. On days zero, 14, and 28, blood samples were obtained for leucocyte phenotyping and phagocytosis assays. On days zero and 28, a lymphoproliferation test, quantification of reactive oxygen (HO) and nitrogen (NO) intermediate production, evaluation of faecal immunoglobulin A (IgA) content, and a delayed cutaneous hypersensitivity test (DCHT) were performed. Statistical analyses were performed with SAS software. Repeated measure variance analyses were performed, and means were compared by the Tukey test. Values of P ≤ 0.05 were considered significant, and values of P ≤ 0.10 were considered tendencies. Dogs fed T400 tended to have higher neutrophilic phagocytic activity than dogs fed T800 (P = 0.073). Regarding reactive oxygen intermediates, bacterial lipopolysaccharide (LPS)-stimulated neutrophils from animals that were fed T400 had a tendency to produce more HO than those from animals fed the control diet (P = 0.093). Elderly dogs, when compared to adult dogs, had lower absolute T and B lymphocyte counts, lower auxiliary T lymphocyte counts, and higher cytotoxic T lymphocyte counts (P < 0.05). A significant effect of diet, age, and time with saline inoculation was noted for the DCHT. There was no effect of diet or age on faecal IgA content in dogs. This study suggests beneficial effects of mannoproteins on the specific and nonspecific immune responses in adult and elderly dogs.

Selenium (Se) is a non-metallic trace element essential for normal cellular function, which has been linked with reduced risk of cancer, cardiovascular disease, cognitive decline and thyroid disease in humans. Se deficiency in livestock is associated with white muscle disease, retained placenta, ill-thrift and mastitis. Where Se status or bioavailability from the soil for plants is poor, livestock rely on supplemental Se in their diets predominantly as either sodium selenite (inorganic form) or selenised-yeast (organic form). As lactic acid bacteria (LAB) have been shown to incorporate Se as either organic or elemental (Nano-Se) there may be potential to use silage inoculant bacteria to improve the Se status of feed to provide the Se requirements of livestock. We screened twenty-seven LAB in MRS broth in the presence of sodium selenite for growth and uptake of Se as organic (selenocysteine and selenomethionine), inorganic (selenite and selenate) or/and Nano-Se, with the aim to identify potential candidates for a mini-silo study. Sodium selenite addition into the growth medium of LAB reduced growth rates but also resulted in the conversion of the inorganic sodium selenite into predominately Nano-Se and small quantities of organic-Se. Based on a rank analysis of growth and ability to take up (total Se content) and convert inorganic Se (Nano and organic Se content), three LAB were selected for further investigation as silage inoculants: DSMZ (A), LF1 (B), and SSL MC15 (C). Each LAB was used as an inoculant within a grass mini-silo trial, either cultured in the presence of sodium selenite before inoculation or sodium selenite added to the inoculum at inoculation versus controls with no Se. The addition of sodium selenite either into the growth media of LAB or applied at inoculation of grass silage did not interfere with the ability of the LAB to act as a silage inoculant with no difference in silage fermentation characteristic between LAB with no Se added. The addition of sodium selenite either to the LAB growth medium or at inoculation resulted in the conversion of sodium selenite into Nano-Se and organic-Se (Nano-Se, ca. 10 higher than organic), as previously shown in the screening trial. There was no difference between the three LAB for incorporation of Se or in silage quality, indicating the potential to develop silage inoculants to increase the bioavailable form of Se (elemental and organic) to livestock through conversion of inorganic forms during ensiling.

Selenium is an essential nutrient for poultry and pigs, and is important for a number of physiological processes including regulation and function of the immune system. Through its incorporation into selenoproteins, Se is involved in the regulation of oxidative stress, redox mechanisms, and other crucial cellular processes involved in innate and adaptive immune response. This review provides current knowledge on the mechanisms by which selenium can modulate the resilience to infectious diseases, and how this micronutrient can influence the capacity of the bird or the pig to maintain its productivity during an infectious challenge. In relation to the most frequent and economically important infectious diseases in poultry and pig production, the present paper considers the influence of different selenium sources (organic inorganic Se) as well as dietary concentrations on the immune responses of poultry and pigs with major emphasis on the potential beneficial impact on animal resilience to common infectious diseases.

The objective of this study was to evaluate the digestible energy (DE), metabolizable energy (ME) content, apparent total tract digestibility (ATTD) of nutrients in chili meal (CM), and to determine the effects of CM on the performance of growing pigs. In Exp. 1, 12 barrows (Duroc x Landrace x Yorkshire) with an initial body weight (BW) of 50.9 ± 1.8 kg were allocated to one of two treatments, corn-soybean meal basal diet or diet containing 194.2 g/kg CM, which replaced corn and soybean meal in the basal diet. Pigs were placed in metabolism crates for a 7-d adaptation period followed by a 5-d total collection of feces and urine to detect DE, ME and ATTD of nutrients in CM. Exp. 2 was conducted for 4 wk. to evaluate the effect of CM on performance of growing pigs. 150 growing pigs (58.4 ± 1.2 kg BW) were allocated to 1 of 5 treatments. Treatment 1 was a corn-soybean meal basal diet met the DE requirement for growing pigs recommended by NRC (2012). Treatment 2 or 3 were diets containing 50 g/kg or 100 g/kg CM respectively.

Diarrhea, resulting from gastrointestinal infection by pathogens, is a common cause of the high mortality and morbidity of neonatal calves. The objective of this study was to evaluate the effects of supplementing a yeast product in milk replacer (MR) on growth and health of calves, and on fecal populations of some targeted microorganisms related to calf health and growth (, total bacteria, , cluster XIVa, and spp.). We hypothesized that feeding a var (SCB) product would improve gastrointestinal health and growth performance of calves. Forty-two Holstein bull calves (42.6 ± 0.77 kg at birth) were randomly assigned on day 2 of age to either a control or SCB treatment. The SCB was supplemented in MR and fed at 5 g/d per head to supply 10 billion colony-forming units per day. All calves received high quality colostrum (>50 mg/mL of immunoglobulin G) during the first 24 h of life, and were fed with 8 L MR (150 g/L mixed with 40 °C water) daily from day 2-35, and 4 L daily from day 35-42. Calves were also fed calf starter from day 7-56. Daily MR and starter offered and refused, daily fecal scores, nasal scores, ear scores, and weekly body weight of calves were recorded. Fecal samples were collected on day 7, 35 and 56 after the first feeding of that day for microbial targets analysis. Overall, there is no serious disease challenge for all the calves during the entire experimental period. No differences were observed in MR intake, starter intake, metabolizable energy (ME) intake, average daily gain, ME intake to gain ratio, fecal score, nasal score, eye score or any targeted microorganisms between treatments throughout the experiment. These results suggest that supplementing SCB in MR has no additive effects on animal growth or fecal biomarkers of gut health when calves do not show deteriorated health status.

The historical background of the present UK legislation is briefly reviewed, and the current legal situation as it pertains to the UK and European Community is summarised. The reasons for the inclusion of antibiotics in feed are outlined and discussed. In the first case, feedstuffs may have to be medicated for reasons of disease prevention and control. In large herds, especially in loose housing, mass medication by injection is not possible and even minimal disease herds will become infected with airborne pathogens from time to time. In the second case, continuous low-level feeding of antibiotics to growing pigs (growth promoters) suppresses the growth of harmful bacteria and allows healthier pigs to grow faster and more efficiently, thus producing cheaper food at the same time as improving welfare. The legal requirements regarding medicines available only on prescription are outlined and discussed. The fears and worries of the general public are noted and found to be overstated because of misconceptions and misunderstanding. It is concluded that food and milk produced today will remain safe provided the safety rules are adhered to.

Fifty Belgian white-blue male calves, purchased from the market at about 10 days of age, were divided into three groups. The basic diet was the same for all calves, and consisted of a restricted amount of commercial milk replacer containing 50 ppm zinc bacitracin and 20 pm virginiamycin, calf starter and grass hay. Weaning occurred when daily starter intake over 7 consecutive days averaged 0.5 kg. Maximum starter intake was restricted to 3 kg day, and grass hay was fed ad libitum. The experiment lasted 20 weeks. Group 1 (control) received the basic diet. Groups 2 and 3 were supplemented with either 2 g purified immunoglobulins (Ig) or 25-ml pooled colostrum (CO) twice daily, via the milk replacer as used in Group 1. Neither Ig nor CO exerted a positive effect on growth rate and feed efficiency. Six calves died: two, one and three in Groups 1, 2 and 3, respectively. Their serum IG content was significantly lower than that of the surviving animals. Mechanisms explaining the lack of any protective effect are discussed. It is supposed that the main reason was due to the gap between the CO feeding at birth and the onset of the administration of Ig or CO at the start of the experiment. At that time, 31 of the 50 calves excreted rota-and/or coronaviruses.