This study investigated the efficacy of organic soil amendments: bokashi (Bok), biochar (BC), and their combination (Bok_BC) in promoting soil health, nutrient availability, and growth of Carrizo citrange (X sp. Rutaceae, Parentage × ) under indoor greenhouse settings. Results indicate significant alterations in soil parameters like total carbon (C), total nitrogen (N), and C:N ratio due to Bok, BC, and Bok_BC treatments. BC treatments boosted total C, while Bok increased total N, compared to controls. A note-worthy 25 % average decrease in C:N ratio was observed with Bok and Bok_BC, nearing the optimal 24:1 C:N for microbial growth. This highlights the potential of waste by-products in balancing nutrient release to benefit soil health and plant development. Analysis of nitrite (NO2-), nitrate (NO3-), and ammonium (NH4-N) levels revealed a dynamic relationship between soil treatments and time. Bok and Bok_BC amendments combined with both fertilizer doses [700 and 1400 Electrical Conductivity, EC] showed an initial NH4-N spike (averaging 1513 and 1288 μg N/g dry, respectively), outperforming control soils (average 503 μg N/g dry). Other key elements like phosphorus, potassium, calcium, and chlorine also experienced initial surges in Bok and Bok_BC soils before declining, suggesting a gradual nutrient release. The concentration of potentially toxic elements remained mostly stable or inconclusive, warranting further exploration. Bok, BC, and Bok_BC treatments considerably influenced germination rate and plant growth. The germination rate averaged 24.2 %, 23 %, and 22.5 % for Bok, BC, and Bok_BC, compared to the 15.9 % control. Plant height increased with Bok, BC, and Bok_BC to 18.4 cm, 18.7 cm, and 16.4 cm, respectively, from the 14.8 cm control. The results remained consistent across fertilizer doses, emphasizing the soil amendments' role in bolstering soil and plant health. In summary, the research underscores the potential of carbon-based amendments like bokashi and biochar in enhancing soil health, reducing reliance on synthetic fertilizers, and fostering sustainable soil ecosystems. The insights are pivotal for advancing sustainable agriculture in indoor greenhouse settings for nursery plant production.

There are many different types of systems used to grow food that are distinguished by ideology or the technology used. It is often difficult to directly compare yield and quality in different growth systems due to the complicated interactions between genotype, physiology and environment. Many published comparisons do not identify and acknowledge confounding factors. However, there is urgency to undertake controlled comparisons to identify the most efficient and effective food production systems, because the world faces considerable challenges to food supply with population rise, ongoing environmental degradation and the threat of climatic change. Here we compared soil with two hydroponic growth systems, drip irrigation and deep-water culture (DWC). It is often claimed that such systems differ in water use, yield and crop quality; however, such comparisons are often confounded by assessing plant and system parameters in different growth environments or where factors that are difficult to standardise between systems, such as nutrient status, are not controlled. We grew tomato ( L.) in the three growth systems in two replicated experiments, in either a polytunnel or glasshouse. We controlled and monitored water use and nutrient levels across all systems as different fertilizer applications can influence the nutritional values of produce. Plants in the two hydroponic systems transpired less water and were more water-efficient with a lower product water use than plants grown in soil. Fruit yield was similar and total soluble solids and sugar levels were not significantly different between the three growing systems. However, levels of lycopene and β-carotene were either similar or significantly higher in DWC compared to growth systems using soil or drip irrigation. Our results identify hydroponic systems as more water use efficient with DWC also capable of producing higher quality produce.

In cassava ( Crantz), transferring genes via genetic breeding depends on crosses between contrasting progenitors, which is often limited by the low flowering rate of many genotypes. The main purpose of this work was to evaluate the effect of grafting on floral induction of cassava. For this, three genotypes were used: 1) BRS Formosa: a genotype with low flowering rate; 2) BGM0823: a genotype with high flowering rate; and 3) FLA05-02: a genotype of ssp. with high flowering rate. Cleft grafting was performed to generate the following treatments: Self-grafting of: 1) BGM0823 (Self-0823); 2) BRS Formosa (Self-Formosa); and 3) FLA05-02 (Self-FLA); and grafting of the genotypes, with the first being the scion and the second the rootstock: 4) BGM0823 × BRS Formosa; 5) BGM0823 × FLA05-02; 6) FLA05-02 × BRS Formosa; 7) FLA05-02 × BGM0823; 8) BRS Formosa × BGM0823; 9) BRS Formosa × FLA05-02; and also ungrafted treatments: 10) BGM0823; 11) BRS Formosa; and 12) FLA05-02. The results showed a 201% increase in the production of male flowers, 560% of female flowers and 400% of fruits in BRS Formosa grafted on BGM0823. BGM0823 (rootstock) also increased fruit production by 190% of FLA05-02. The grafted cassava plants exhibited an increase on the shoot production, although there was no change in the fresh root yield. The grafting of genotypes with high flowering rates can induce flowering in genotypes with low flowering rates.

This paper deals with vegetable cultivation that could be faced in a space mission. This paper focusses on optimization, light, temperature and the harvesting process, while other factors concerning cultivation in space missions, i.e. gravity, radiation, were not addressed. It describes the work done in preparation of the deployment of a mobile test facility for vegetable production of fresh food at the Neumayer III Antarctic research station. A selection of vegetable crops was grown under varying light and temperature conditions to quantify crop yield response to climate factors that determine resource requirement of the production system. Crops were grown at 21 °C or 25 °C under light treatments varying from 200 to 600 μmol m  s and simulated the dusk and dawn light spectrum. Fresh food biomass was harvested as spread harvesting (lettuce), before and after regrowth (herbs) and at the end of cultivation. Lettuce and red mustard responded well to increasing light intensities, by 35-90% with increasing light from 200 to 600 μmol m s, while the other crops responded more variably. However, the quality of the leafy greens often deteriorated at higher light intensities. The fruit biomass of both determinate tomato and cucumber increased by 8-15% from 300 to 600 μmol m s. With the increase in biomass, the number of tomato fruits also increased, while the number of cucumber fruits decreased, resulting in heavier individual fruits. Increasing the temperature had varied effects on production. While in some cases the production increased, regrowth of herbs often lagged behind in the 25 °C treatment. In terms of fresh food production, the most can be expected from lettuce, cucumber, radish, then tomato, although the 2 fruit vegetables require a considerable amount of crop management. Spread harvesting had a large influence on the amount of harvested biomass per unit area. In particular, yield of the 3 lettuce cultivars and spinach was ca. 400% than single harvesting. Increasing plant density and applying spread harvesting increased fresh food production. This information will be the basis for determining crop growth recipes and management to maximize the amount of fresh food available, in view of the constraints of space and energy requirement of such a production system.

Drought is a major factor limiting crop production worldwide. The objective of this study was to test whether pre-exposure to drought can enhance the subsequent drought response of a drought-sensitive variety of olive cv. . Seven-months old olive plants were grown in a controlled conditions and divided into control plants (irrigated daily), primed plants (PP, primed by exposure to drought for 21 days, re-watered for 60 days and then exposed to water depletion for 30 days) and non-primed plants (NPP, well watered for 81 days and immediately followed by intermediate drought as PP). Compared to the non-primed plants, primed plants showed an improvement in biomass production and healthy values of photosynthesis parameters with a higher accumulation of photosynthetic pigments. Additionally, the data of chlorophyll fluorescence were significantly similar to those of control, implying that no photodamage was occurred. Moreover, primed plants exhibited high accumulation of total sugar and proline which lead to the better water status maintenance. The lower level of oxidative status measured in term of hydrogen peroxide (HO), malondiadehyde (MDA) and electrolyte leakage (EC) in primed plants confirmed the alleviation of oxidative stress. Furthermore, the primed plants possessed more effective oxygen scavenging systems as exemplified by the increased activities of CAT, SOD, GP and high accumulation of polyphenols, resulting in a better maintenance in homeostasis of ROS production. Our investigation is indicative of the result of the benefit memory effects caused by stress pre-exposure in young olive plants cv.'' to overcome subsequent stress.

An efficient protocol for hairy root induction of spine gourd () was established using (KCTC 2703). This study evaluates the phenolic compound production, antioxidant and antimicrobial (antibacterial, antifungal and antiviral) activities of transgenic hairy root cultures in . Hairy roots were induced from leaves, petiole, and internodal explants. Molecular analysis of PCR and gene sequencing using specific primers of and revealed T-DNA integration in the hairy root clones and RT-PCR analysis confirmed the expression of hairy root inducible genes ( and ). The greatest biomass accumulation of hairy roots on MS liquid medium supplemented with 3% sucrose was observed at 22 days. Ultra-HPLC was used to compare the individual phenolic compound contents of transgenic and non-transgenic roots. Moreover, transgenic hairy roots efficiently produced several phenolic compounds, such as flavonols, hydroxycinnamic acid and hydroxybenzoic acid derivatives. The total phenolic, flavonoid contents and biological (antioxidant, antibacterial, antifungal and antiviral) activities were higher in hairy roots compared to non-transformed roots. These results demonstrate the greater potentiality of hairy root cultures for the production of valuable phenolic compounds and for studies of their biological activity.

Grapevine, an important horticultural crop in the world, is moderately tolerant to cold conditions and is subjected to the cold injuries at different regions. So studies on different aspects of tolerance mechanism to unexpected cold of late spring as well as winter freezing seems necessary about this vine. For this reason, study on genes responsible for acquiring cold tolerance is very important. Transcription factors are among regulatory proteins that are responsible for cold acclimation. In this research work, expression levels of CBF1, CBF2, CBF3, and CBF4 transcription factors were studied on two cvs of ("Khalili-Danedar" and "Shahroodi") as well as one at different times after treating at 4 °C. Results showed that two cultivars, "Khalili-Danedar" and "Shahroodi", had similar trend for each transcription factor. Gene expression increased at the beginning of cold stress and then decreased. Expression of these TF started some minutes (CBF1) after cold treatment and continued for several hours (CBF2), even till the tenth day (CBF4). All together which is endemic to the cold regions behaved stronger and showed higher expression for all studied transcription factors. Among two cultivars, "Khalili-Danedar" showed significantly higher expression compared with "Shahroodi". The comparison of expression levels of these four transcription factors revealed that the least and the greatest expressions were recorded for CBF1 and CBF3 respectively, and two CBF2 and CBF4 had approximately the same expression levels.

High-temperature and waterlogging are major abiotic stresses that affect the yield and quality of cauliflower. Cauliflower cultivars 'H41' and 'H69' are tolerant to high temperature and flooding, respectively; however, 'H71' is sensitive to both stresses. The objectives of this study were to identify the proteins that were differentially regulated and the physiological changes that occurred during different time periods in 'H41', 'H69', and 'H71' when responding to treatments of flooding, 40 °C, and both stresses combined. Changes in the leaf proteome were analyzed by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS) and identified by Mascot peptide mass fingerprint (PMF) and database searching. Stress treatments caused significant reductions in electrolyte leakage, chlorophyll fluorescence Fv/Fm, chlorophyll content, and water potential as stress times were prolonged. By the comparative proteomic analysis, 85 protein peaks that were differentially expressed in response to combination treatments at 0, 6, and 24 h, 69 (33 in 'H41', 29 in 'H69', and 9 in 'H71') were identified, of which were cultivar specific. Differentially regulated proteins predominantly functioned in photosynthesis and to a lesser extent in energy metabolism, cellular homeostasis, transcription and translation, signal transduction, and protein biosynthesis. This is the first report that utilizes proteomics to discover changes in the protein expression profile of cauliflower in response to heat and flooding.

Flour derived from Cycas micronesica seeds was once the dominant source of starch for Guam's residents. Cycad consumption has been linked to high incidence of human neurodegenerative diseases. We determined the distribution of the sterols stigmasterol and β-sitosterol and their derived glucosides stigmasterol β-d-glucoside and β-sitosterol β-d-glucoside among various plant parts because they have been identified in cycad flour and have been shown to elicit neurodegenerative outcomes. All four compounds were common in seeds, sporophylls, pollen, leaves, stems, and roots. Roots contained the greatest concentration of both free sterols, and photosynthetic leaflet tissue contained the greatest concentration of both steryl glucosides. Concentration within the three stem tissue categories was low compared to other organs. Reproductive sporophyll tissue contained free sterols similar to seeds, but greater concentration of steryl glucosides than seeds. One of the glucosides was absent from pollen. Concentration in young seeds was higher than old seeds as reported earlier, but concentration did not differ among age categories of leaf, sporophyll, or vascular tissue. The profile differences among the various tissues within these organs may help clarify the physiological role of these compounds.

An in vitro culture system was used to study the influence of gravity on axillary shoot formation and adventitious root regeneration in Mentha piperita L. The direction of the gravity vector was altered by displacing stem node explants in different orientations. Also, microgravity conditions were simulated by rotating the explants on a horizontal clinostat so that the main axis of nodes was either parallel (Cpa) or perpendicular to the clinostat axis (Ccp and Ccf, centripetally and centrifugally oriented, respectively). Mint nodes were cultured on solidified Linsmaier and Skoog's medium [Physiol. Plant. 18 (1965) 100] adding a filter-sterilized aqueous solution of 2 mg/l benzyladenine (BA) in half of the cultures. The proliferation of axillary shoots as well as adventitious root formation were not affected by altering upright explant orientation. On the contrary clinorotation was able to modify plantlet development. In absence of BA, leaf width was hindered by Cpa treatment and penultimate internode length was enhanced by Ccp. Furthermore, a negative effect of Cpa treatment was observed in root length parameter, while Ccp increased the root number both in absence and in presence of BA. An effect strictly connected to clinorotation in presence of BA was the occurrence of hyperhydricity. Moreover, explants under clinorotation treatments switched their gravitropic response modifying shoot curvature.

'Waldmann's Green' leaf lettuce (Lactuca sativa L.) was grown either under 84% irradiance from incandescent (In) + 16% from fluorescence (Fl) lamps, or 100% from metal halide (MH) lamps, both at 392 micromoles s-1 m-2 of photosynthetically active radiation (400-700 nm) from 11 to 19 days after seeding. No differences in leaf dry weight, leaf area, relative growth rate (RGR) or photosynthesis (Pn) occurred after 8 days of exposure to these radiation treatments for 20 h day-1. However, a 23% reduction in root dry weight, a 123% increase in stem length and a 61% increase in stem dry weight were found with In + Fl relative to MH radiation. A photosynthetic photon flux (PPF) of 920 micromoles s-1 m-2 from the In + Fl source increased leaf dry weight by 13% and RGR by 21% relative to those at 460 micromoles s-1 m-2 from the same source. From 4 to 8 days of treatment, high PPF did not lower shoot dry gain, but did lower RGR. Photosynthesis and net assimilation rate were lower while leaf area ratio was higher at 460 than at 920 micromoles s-1 m-2 over the 8-day treatment period. When PPF from MH lamps was 400 micromoles s-1 m-2, leaf dry weight was 20% greater than if PPF was 805 micromoles s-1 m-2 after 4 days of treatment, but no differences were detected after 8 days. Relative growth rate increased by 11% during the first 4 days, but declined by 12% during the second 4 days under high- relative to low-PPF MH radiation. Lettuce productivity was stimulated by high PPF from either lamp type from Day 11 to Day 14, but not from Day 15 to Day 19 after seeding.

Container-grown eggplant (Solanum melongena L. var esculentum Nees. 'Burpee's Black Beauty') seedlings were conditioned with brief, periodic mechanical stress or abscisic acid (ABA) in a greenhouse prior to outdoor exposure. Mechanical stress consisted of seismic (shaking) or thigmic (stem flexing) treatment. Exogenous ABA (10(-3) or 10(-4)M) was applied as a soil drench 3 days prior to outdoor transfer. During conditioning, only thigmic stress reduced stem elongation and only 10(-3) M ABA reduced relative growth rate (RGR). Both conditioning treatments increased leaf specific chlorophyll content, but mechanical stress did not affect leaf ABA content. Outdoor exposure of unconditioned eggplant seedlings decreased RGR and leaf-specific chlorophyll content, but tended to increase leaf ABA content relative to that of plants maintained in the greenhouse. Conditioning did not affect RGR of plants subsequently transferred outdoors, but did reduce stem growth. Seismic stress applied in the greenhouse reduced dry weight gain by plants subsequently transferred outdoors. Mechanical stress treatments increased leaf water potential by 18-25% relative to that of untreated plants.

Effects of different ratios incandescent (ln) to fluorescent (Fl) radiation were tested on growth of 'Waldmann's Green' leaf lettuce (Lactuca sativa L.) in a controlled environment. After 4 days of treatment, dry weight, leaf area, relative growth rate (RGR), net assimilation rate (NAR), leaf area ratio (LAR) and photosynthetic rate (Pn) were greater for plants grown at 84 rather than 16% of total irradiance (82 W m-2) from ln lamps. Although leaf dry weight and area were 12-17% greater at 84% ln after the first 8 days of treatment, there were no differences in RGR or Pn between treatments during the last 4 days. If 84% ln was compared with 50% ln, all cumulative growth parameters, RGR, NAR and Pn were greater for 84% ln during the first 4 days of treatment. However, during the second 4 days, RGR was greater for the 50% ln treatment, resulting in no net difference in leaf dry weight or area between treatments. Shifting from 84 to 50% ln radiation between the first and second 4 days of treatment increased plant dry weight, leaf area, RGR and NAR relative to those under 84% ln for 8 days continuously.