Ethylene has an essential function in the biosynthesis of anthocyanin. However, the molecular mechanism through which ethylene impacts the color of lily flower remains little appreciated. This study identified LhERF061, a dehydration-responsive element-binding (DREB family) transcription factor that suppresses anthocyanin biosynthesis in response to ethylene in lilies. Transient LhERF061 overexpression caused a dramatic decrease in anthocyanin levels and downregulated both anthocyanin structural genes and positive regulators in lily tepals. Heterologous LhERF061 expression in Arabidopsis and tobacco also suppressed anthocyanin accumulation. Mechanistically, LhERF061 was found to bind to the promoters of LhMYBSPLATTER (a positive regulator of the biosynthesis of anthocyanin) and LhDFR (an anthocyanin structural gene), thereby inhibiting their transcriptional activity. Further investigation indicated that LhERF061 physically interacted with LhMYBSPLATTER, thereby interfering with the MYB-bHLH-WD40 (MBW) complex responsible for anthocyanin regulation, providing multiple mechanisms for inhibiting the biosynthesis of anthocyanin. These results provide insights into how ethylene mediates the biosynthesis of anthocyanin and increase understanding of the regulatory network of the biosynthesis of anthocyanin in lily.

To date, only a few submerged plants have been reported to perform C and CAM. Ottelia cordata is a heteroblastic aquatic plant developing both submerged and floating leaves throughout its life cycle. Previous research found that, besides HCO use, the submerged leaves of O. cordata can also perform C metabolism. However, it remains unclear how the HCO use or the C pathway, and the anatomical structure, respond to varying CO environments. Therefore, we examined the anatomical structures and carbon-concentrating mechanisms in O. cordata submerged leaves under high (HC) and low CO (LC) conditions. Our results revealed the leaf consists of an upper and lower layer of epidermal cells, separated by large air spaces and two layers of mesophyll cells, without the presence of Kranz anatomy. Both epidermal and mesophyll cells contained chloroplasts, but starch grains were larger in the mesophyll chloroplasts than in the epidermal cells. Additionally, the area of the upper epidermal cells, mesophyll cells, air spaces, chloroplast, and starch grains significantly increased under HC. Moreover, the ability to utilize HCO was stronger under LC. The activity of photosynthetic enzymes, kinetics of O evolution, and δC confirmed the C pathway under both HC and LC. However, the organic acid results indicated that CAM was not operational in either HC or LC. In summary, our findings suggested that the ability to use HCO and a C-like metabolism may occur in the submerged leaves of O. cordata, despite the absence of Kranz anatomy, across both HC and LC environments.

This study was carried out to quantify changes in the activities of antioxidative enzymes such as catalase (CAT), peroxidase (POX) and superoxide dismutase (SOD) in two resistant onion cultivars 'Saba - HS' and 'Saba' and two susceptible cultivars 'Golden Eye' and 'Savannah Sweet' following an inoculation with Fusarium oxysporum f. sp. cepae (FOC) at seedling stage. Further, we assessed the expression of transcription factors (TFs), R1, PR5 and RGA29-genes that are involved in conferring resistance post-inoculation using qRT-PCR. The results revealed that the least disease severity occurred with the resistant 'Saba - HS' (4.7%) and 'Saba' (6.7%) cultivars, whereas the highest disease severity occurred with the susceptible 'Golden Eye' (89.6%) and 'Savannah Sweet' (88.9%) cultivars, respectively. Both resistant genotypes 'Saba' and 'Saba-HS' showed a significant increase in CAT, POX, and SOD activities at a transcriptional level. CAT activity was upregulated 4.81-fold in 'Saba' and 4.22-fold in 'Saba-HS' followed by POX where 'Saba' showed an increase by 3.53-fold and 'Saba-HS' by 2.35-fold, and SOD 'Saba' 17.46-fold and 'Saba-HS' 22.95-fold, relatively. Marker genes, RGA29, R1 and PR5, were also upregulated in the resistant-genotypes by 3.83, 4.78 and 5.01-fold, in comparison to their-controls, respectively. Similar trends were recorded for the biomass growth parameters (BGPs).

Combining imidazolinone-tolerant wheat with imazamox presents an effective solution to combat weed resistance. However, Lolium multiflorum, a troublesome resistant weed infesting wheat fields, may have developed resistance to imazamox, and the potential resistance mechanisms are intriguing. In this study, we explored the susceptibility of L. multiflorum to imazamox and investigated the resistance mechanisms, including the contribution of the target enzyme acetolactate synthase (ALS) to resistance and the presence of non-target-site resistance (NTSR). Eight L. multiflorum populations suspected of being resistant to imazamox were collected, and six populations exhibited resistance, ranging from 2.45-fold to 16.32-fold. The LmALS1 gene from susceptible population D3 plants and multiple copies of the LmALS gene (LmALS1, LmALS2, LmALS2α, LmALS3, LmALS3α, LmALS3β) from resistant populations D5 and D8 plants were separately amplified. Two mutations (Pro/Gln197 to Thr, Trp574 to Leu) were found in LmALS1 in the resistant populations. Compared to D3, LmALS1 was overexpressed in D5 but not in D8. The presence of LmALS1 mutants (LmALS1-Thr197 and LmALS1- Leu574), along with LmALS2, LmALS3, and their subunits, contribute to the resistance phenotype by increasing bonding energies, weakening hydrogen bonds, or decreasing protein binding pocket volumes and surface area. Additionally, D5 and D8 populations exhibited multiple resistance (>40-fold) to three other ALS inhibitors: pyroxsulam, flucarbazone-sodium, and mesosulfuron-methyl. Pre-treatment with malathion and 4-chloro-7-nitrobenzoxadiazole (cytochrome P450 monooxygenase and glutathione S-transferase inhibitors respectively) reversed the resistance of the D8 population and partially reversed the resistance of the D5 population to imazamox. This study characterizes ALS genes and extends our knowledge into the ALS resistance mechanisms involved in L. multiflorum. It also deepens our understanding of the complex diversification resistance mechanisms, thereby facilitating advances in weed resistance management strategies in wheat fields.

Tobacco (Nicotiana tabacum L.) plants were grown outdoors (N°46.07, E°18.18) under either natural or UV-deprived sunlight for 25 days in the summer. High PAR resulted in high polyphenol content, which was selectively affected by solar UV-A and UV-B irradiation. Solar UV-A irradiation increased anthocyanins, but not flavonoids, in the epidermis, and this additional protection resulted in higher photochemical yields and lower NPQ. The simultaneous presence of UV-B overrode the effects of UV-A, increased epidermal flavonoids, and decreased anthocyanins. Leaves grown in full sunlight had the same photochemical yields of NPQ as those grown under a UV-excluding filter. A combination of these effects can falsely dismiss the effects of UV-B on outdoor photosynthesis. Phenolic acid content, corresponding to approximately 80% of phenolic compounds, did not depend on solar UV, and total flavonoids increased under full solar UV irradiation, but not under UV-A only. The polyphenol content in outdoor leaves also served as a reference point for an indoor experiment, which showed that even a short, 4-day exposure of low PAR grown plants to UV from an artificial source increased the amount of some, although not all, components close to or even above outdoor levels. In indoor leaves, a selective increase in quercetin glycosides (to 62-85% of outdoor levels) supports both enzymatic and non-enzymatic antioxidant functions, and the increase in crypto- and neochlorogenic acids (to 76% and 117% of outdoor levels, respectively) suggests a redistribution among biosynthesis pathways. These results demonstrate the potential and efficiency of cultivation systems without sunlight.

Salt-drought is a major environmental event affecting crop productivity and quality by causing chlorophyll (Chl) and carbon balance disorder. There has been growing interest in the application of endophyte and silicon (Si), as inoculants for saline and drought land restoration. This study investigates the impact of Bacillus (Bs), Si, and Bs + Si on the disorder of Chl metabolism and carbon balance of G. uralensis seedlings under salt-drought stress (SD). Results showed that both Bs and Si treatments enhanced Chl and carbon metabolism, with the combined Bs and Si treatment showing a synergistic effect. Specifically, Bs + Si enhanced the mutual conversion of Chl a and Chl b, restored the equilibrium in Chl a and Chl b content, and increased RuBisco activity by 31.07%, thereby promoting carbon fixation. Subsequently, Bs + Si re-balanced the carbohydrate content, by increasing the sucrose synthase (SS), and β-amylase (BMY) activities by 49.57%, and 83.59% respectively, and decreasing sucrose phosphate synthase (SPS), and granule-bound starch synthase (GBSS) activities by 38.93%, 40.93% respectively etc involved in the metabolism of sucrose and starch. Furthermore, Bs + Si facilitated the restoration of the typical progression of the tricarboxylic acid (TCA) cycle and glycolysis pathway (EMP). These findings highlight the synergistic role of Bs and Si in enhancing the salt and drought resilience of G. uralensis seedlings, offering promising strategies for sustainable agriculture, improving crop resilience to climate change, and achieving the "dual carbon" goals of carbon peaking and carbon neutrality.

Natural Resistance-Associated Macrophage Protein (NRAMP), a class of metal transporter proteins widely distributed in plants, is mainly involved in the uptake and transport by plants of metal ions, such as iron, manganese and cadmium. The current study is the first to fully investigate the Triticum aestivum (T. aestivum) NRAMP gene family. 33 NRAMP members were identified from the entire T. aestivum genome and classified into three main groups based on related genes found in five other species. Among the TaNRAMP genes, the exon-intron structure and motif composition exhibited significant similarity among members of the same evolutionary branch of the phylogenetic tree. Based on RNA-seq and qRT-PCR analyses, we identified the expression patterns of the TaNRAMP genes in different tissues and under various stress conditions. TaNRAMP genes expression were responsive to induction by cadmium (Cd). Overexpression of the TaNRAMP5 gene enhanced wheat and tobacco tolerance to Cd toxicity. Additionally, the TaNRAMP5 protein physically interacted with protein phosphatase 2A (PP2A) in yeast cells. This study provides a valuable reference point for further investigations into the functional and molecular mechanisms of the NRAMP gene family.

Blue light simultaneously enhances anthocyanin and carotenoid biosynthesis in mango (Mangifera indica L.) fruit peel and flesh, respectively, but the mechanism remains unclear. In this study, two blue light-triggered zinc-finger transcription factors, MiBBX24 and MiBBX27, that positively regulate anthocyanin and carotenoid biosynthesis in mango fruit were identified. Both MiBBXs transcriptionally activate the expression of MiMYB1, a positive regulator of anthocyanin biosynthesis. Furthermore, both MiBBXs also trigger the expression of a phytoene synthase gene (MiPSY), which is essential for carotenoid biosynthesis. Ectopic expression of MiBBX24 or MiBBX27 in Arabidopsis increased anthocyanin contents, and their positive effects on anthocyanin accumulation in mango peel were confirmed through transient overexpression and virus-induced silencing. Transient expression of MiBBX24 or MiBBX27 in tomato (Solanum lycopersicum) and mango fruit flesh increased the carotenoid content, while the virus-induced silencing of MiBBX24 or MiBBX27 in the mango fruit flesh decreased carotenoid accumulation. Overall, our study results reveal that MiBBX24 and MiBBX27 simultaneously promote the biosynthesis of anthocyanin and carotenoids biosynthesis in mango fruit peel and flesh under blue light, indicating that BBX-mediated dual effects on physiological functions contribute to mango fruit pigment accumulation. Furthermore, we herein shed new light on the simultaneous transcriptional regulatory effects of a single factor on the biosynthesis of different plant pigments.

Soil water deficit is a key environmental factor limiting peanut yield and quality, which can occur at any growth stage of peanut. But the exact mechanism of soil water deficit affecting the formation of peanut yield and quality remains unclear. In this study, the seed development, yield components, oil accumulation and fatty acid composition of common (HY25; FH18) and high oleic acid varieties (KN71; HY52) under soil water deficit throughout the growth period were investigated. It was found that the decrease of pod number and 100-pod weight per plant was the main factor leading to the reduction in peanut yield under soil water deficit. The number of oil bodies, maximum oil accumulation rate and oil content were significantly reduced, especially in drought-sensitive peanut varieties. The down-regulation of enzyme activities on the Kennedy pathway was the main factor hindering oil synthesis. Peanut varieties with lower levels of FAD2 transcripts might more sensitive to drought stress in terms of fatty acid metabolism. Under soil water deficiency, high oleic acid peanut oleate synthase activity was reduced, oleic acid metabolizing enzyme activity was elevated, which lead to decreased oleic acid content and the ratio of oleic acid to linoleic acid (O/L), and impaired lipid quality. Among them, the lipid quality of HY52 was most severely compromised. In contrast, the common varieties exhibited opposite enzyme activity patterns, with increases in oleic acid content and O/L, and improved lipid quality. This study elucidated the response mechanism of peanut grain development and oil metabolism to soil water deficit, which can provide theoretical basis and technical support for realizing high quality and stable yield of peanut under adversity.

Abiotic stresses, such as extreme temperatures, drought, and salinity, significantly affect plant growth and productivity. Among these, cold stress is particularly detrimental, impairing cellular processes and leading to reduced crop yields. In recent years, stress-associated proteins (SAPs) containing A20 and AN1 zinc-finger domains have emerged as crucial regulators in plant stress responses. However, the functions of SAPs in tobacco plants remain unclear. Here, we isolated Nicotiana tabacum SAP9 (NtSAP9), whose expression was induced by cold treatment, based on RNA-sequences data. Knock down of NtSAP9 expression reduced freezing tolerance, while overexpression conferred freezing tolerance in transgenic tobacco plants, as indicated by relative electrolytic leakage and photosystem II photochemical efficiency. Untargeted metabolomics via liquid chromatography-tandem mass spectrometry revealed distinct metabolic profiles between WT and NtSAP9-overexpressing tobacco plants under normal and low temperature conditions. Upregulation of amino acids like D-Glutamine, DL-Glutamine, and O-Acetyl-L-serine suggests NtSAP9 enhances cold tolerance. Further expression analysis by quantitative real-time PCR indicated that NtSAP9 participates in cold stress response possibly through amino acid synthesis-related genes expression, such as glutamine synthetase and glutamate dehydrogenase. These findings improve our understanding of SAP proteins in tobacco's response to cold stress.

Petal pigmentation in carnations is closely associated with the biosynthesis of anthocyanins. This biosynthetic process is tightly regulated by transcription factors, which activate or repress key genes involved in anthocyanin production. Here, we aim to explore the mechanisms involved in the transcriptional regulation of anthocyanin biosynthesis in carnation petals. We identified DcWRKY15 as a critical regulator influencing anthocyanin production in these petals. DcWRKY15 expression showed a strong correlation with the expression levels of genes associated with anthocyanin biosynthesis, peaking during early petal development stages. The findings from the dual-LUC, VIGS, Y1H and EMSA assays demonstrated that DcWRKY15 played a positive regulatory role in anthocyanin biosynthesis. DcWRKY15 achieved this by binding directly to the promoters of DcCHS and DcF3H, thereby enhancing their expression. Additionally, DcWRKY15 interacts with the repressor DcMYB2, which reduces its capacity to enhance anthocyanin biosynthesis, particularly during the later stages of petal development. These findings offer new insights into the molecular mechanisms responsible for petal coloration in carnations, highlighting the complex interplay between activator and repressor transcription factors.

This study investigates the impact of neodymium (Nd) nanoparticle (NdNP) toxicity on the physiological and biochemical responses of sorghum (Sorghum bicolor) and oat (Avena sativa) plants and evaluates the potential mitigating effects of arbuscular mycorrhizal fungi (AMF). Sorghum and oat plants were grown under controlled conditions with and without AMF inoculation, and subjected to NdNPs (500 mg Nd kg soil). Results revealed that Nd nanoparticles significantly reduced biomass in both species, with a 50% decrease in sorghum and a 59% decrease in oats. However, AMF treatment ameliorated these effects, increasing biomass by 69% in oats under Nd nanoparticles toxicity compared to untreated contaminated plants. Soluble sugar metabolism was notably affected; AMF treatment led to significant increases in fructose and sucrose contents in both sorghum (+31% and +23%, respectively) and oat (+25% and +37%, respectively) plants under NdNPs toxicity. Improved sugar metabolism via enhanced activities of sucrose phosphate synthase (+29-54%) and invertase (+39-54%) enzymes resulted in higher proline (+21-81%) and polyamines (+49-52%) levels in AMF-treated plants under NdNPs toxicity, along with alterations in the biosynthesis pathways of amino acids and fatty acids, resulting in better osmoprotection and stress tolerance. Moreover, citrate (+29-55%) and oxalate (+177-312%) levels increased in both plants in response to NdNPs toxicity, which was accompanied by a positive response of isobutyric acid to AMF treatment in stressed plants, which potentially might serve as mechanisms for plants to mitigate NdNPs toxicity. These findings suggest that AMF can significantly mitigate Nd-induced damage and improve plant resilience through enhanced metabolic adjustments, highlighting a potential strategy for managing rare earth element (REE) nanoparticle toxicity in agricultural soils.

Cold stress is one of the important harmful factors that seriously affect wheat (Triticum aestivum) yield and quality. TCP transcription factor plays important roles in the process of plant cell proliferation and growth. In this study, we identified 60 TaTCP genes expressed in strong cold resistant winter wheat variety Dongnongdongmai1 (Dn1) under cold stress by previous transcriptome data, of which 13 TaTCPs showed significant differences in expression. The evolution of TaTCPs was analyzed, and the results showed that there were 2 homologous pairs in TaTCPs with AtTCPs and 90 homologous pairs in TaTCPs with OsTCPs. Expression patterns of 20 TaTCPs under cold stress were analyzed by qRT-PCR, and TCP21-A with significant expression differences was screened. We obtained tcp21-A mutant from the EMS mutant library of winter wheat Kenong9204. We observed that the mutation of TaTCP21-A significantly improved its cold resistance. Subsequently, transcriptome analysis revealed that TCP21-A inhibited expression of cold responsive gene TaDREB1C. Finally, subcellular localization and yeast one hybrid were used to verify that TCP21-A can act as a transcription factor to bind to the GGTCCC promoter element. Luciferase reporter gene experiment showed that TCP21-A inhibits the transcriptional activity of the TaDREB1C promoter. In summary, we systematically analyzed the expression patterns of TaTCP family members in Dn1 under cold stress and demonstrated that TaTCP21-A negatively regulated wheat cold tolerance by inhibiting expression of TaDREB1C. These results provide new insights into the functional mechanism of TaTCP transcription factors in response to cold stress.

Ethylene plays crucial roles in the adaptation to cadmium (Cd) stress. Nevertheless, the impact of endogenous ethylene on radial transport of Cd in different rice cultivars are insufficiently understood. Herein, we investigated how ethylene involved in the formation of endodermal barriers in roots of Nipponbare with low-Cd accumulation and IR32307 with high-Cd accumulation ability and further assessed its influence on Cd radial transport. Our analysis indicated that both Cd stress and external ACC (1-aminocyclopropane-1-carboxylic acid, ethylene biosynthesis precursor) promoted the ethylene production. Intriguingly, the positive response of ethylene signal to Cd was more intensive in roots of Nipponbare than that of IR32307. The increased endogenous ethylene in rice roots promoted development of casparian strips (CSs) and suberin lamellae (SL). Specifically, external addition of ACC decreased the percentage of the D/D to root length by 44.4-79.6%/49.3-11.4% in Nipponbare and 18.7-19.9%/10.7-35.3% in IR32307, individually. The intrinsic molecular mechanism was mainly due to changes in the genes expression levels related to CSs/SL biosynthesis. Simultaneously, the analyses of apoplastic tracer (Propidium Iodide, PI) and cell-to-cell tracer (Fluorescein Diacetate, FDA) confirmed that the ethylene-mediated endodermal barriers were functional, which were in accordance with the increased/reduced Cd transport in roots. Eventually, the results of transcriptome analysis further shed a comprehensive insight that ethylene constructed the endodermal barrier through phenylpropanoid and cutin, suberine and wax biosynthesis to reduce Cd radial transport in rice, which are beneficial for the breeding of rice with low-Cd accumulating capacity in the future.

Previous studies have shown that the bacterial fertilizer Lactobacillus reuteri (LBR) significantly alleviates apple replant disease (ARD), but the mechanism behind its effectiveness remains unclear. This study investigated the effects of key LBR metabolites on the rhizosphere microbial community. The biocontrol function of extracellular polysaccharides (EPS) was examined and shown to be further enhanced after optimizing the fermentation conditions. The optimized fermentation conditions were found to generate intermediates involved in various plant metabolic pathways, leading to plant growth promotion, increased abundance of beneficial bacteria like Bacillus and Pseudomonas in the rhizosphere soil, and decreased abundance of pathogenic fungi. Through the isolation and identification of rhizosphere microorganisms, a strain of Pseudomonas monteilii with chemotaxis to EPS was isolated, which had growth promotion ability and effectively improved plant resistance and relieves ARD. To further understand the mechanism underlying the inhibitory effect on soil pathogens of microbial aggregations and development in the rhizosphere driven by beneficial bacteria metabolites. These findings offer valuable technical insights for utilizing biocontrol bacteria metabolites in ARD management.

The escalating utilization of wireless electronic devices, notably cellular phones, has led to a substantial augmentation in the levels of electromagnetic field radiation (EMF-r) within the environment. Consequently, an imperative arises to investigate the impact of these radiations on biological systems, specifically on plants. In this study, we examined the genotoxic and cytotoxic effects of 2100 MHz and 2300 MHz EMF-r on the Trigonella foenum-graecum L. test system, evaluating parameters such as percentage germination, growth characteristics, biochemical activities, cell viability and chromosomal aberrations. The roots and shoots of T. foenum-graecum L. were exposed to 2100 MHz and 2300 MHz EMF-r for varied exposure durations (0.5 h, 1 h, 2 h, 4 h and 8 h/day) (at a power density of 10.0 dBm). Substantial reductions in root and shoot lengths were observed after a exposure period of 4 h and 8 h at a frequency of 2100 MHz and 2300 MHz. Genotoxic studies revealed both physiological and clastogenic effects of EMF-r, as evidenced by an increase in chromosomal aberrations (CAs). Biochemical analyses demonstrated elevated malondialdehyde levels and activities of various antioxidative enzymes following 2 h and 4 h per day exposure to 2100 MHz and 2300 MHz EMF-r. Chromosomal aberrations increased by 2.88%-14.86% and 2.84%-18.49% (0.5 h-8 h per day) exposure to 2100 MHz and 2300 MHz, respectively when compared to that of the control group. Exposure to electromagnetic fields (EMF-r) at both 2100 MHz and 2300 MHz resulted in reduced cell viability. This study examines the impact of electromagnetic fields (EMF-r) at 2100 MHz and 2300 MHz on plant root meristems. The results reveal more pronounced genotoxic effects at 2100 MHz, highlighting the frequency-dependent nature of EMF-r impacts. By providing insights into these frequency-specific effects, this research addresses a critical gap in understanding how EMF-r influences plant cellular health and offers guidance for mitigating potential environmental risks from mobile communication technologies.

Strawberries (Fragaria x ananassa) are valued worldwide for their aroma among other quality traits. Pyruvate decarboxylase (PDC) is a key enzyme in aroma, initiating the conversion of pyruvate into acetaldehyde. This process produces precursors for esters and aromatic compounds that enhance strawberry aroma. Additionally, alcohol acyltransferases (AATs) are essential for catalyzing acyl group transfers, further enriching fruit aroma diversity. However, strawberries are highly vulnerable to drought, which affects product quality. Plant root-associated fungi offer a novel approach to mitigate water deficiency stress. This study investigates the effect of Antarctic fungal inoculation on the gene expression of FaPDC, and the FaAAT gene family, related to the accumulation of volatile organic compounds (VOCs) in strawberries. Fruits of fungi-inoculated plants under drought stress showed significant changes in gene expression, leading to increased total volatile ester production, primarily in acetate esters, which are important for strawberry aroma. These findings underscore the role of Antarctic fungi in modulating the metabolic pathway of volatile esters by inducing the expression of FaPDC and FaAAT genes. Beyond elucidating the molecular mechanisms underlying aromatic compound biosynthesis in fruits, this study highlights the potential of Antarctic microorganisms as valuable tools to restore and maintain the sensory attributes of agricultural products under water deficiency stress.

Intercropping system influences the endophytic microbial abundance, hormone balance, nutrient metabolism and yield, but the molecular mechanism of yield advantage in Camellia oleifera intercropping with peanut is not clear. In this study, the C. oleifera monoculture (CK) and C. oleifera-peanut intercropping (CP) treatments in purple soil were conducted, and the physicochemical properties, gene expressions, signal pathways and crucial microbial abundances were investigated to reveal the molecular mechanism of the yield advantage of intercropped C. oleifera. The results showed that the intercropping system increased in contents of pigment, carbohydrate, available nitrogen and phosphorus in leaf and root, as well as the abundances of Burkholderia, Ralstonia, Delftia, Pseudoalteromonas and Caulobacter, enhanced the relative expression levels of CoSPS, CoGBE, CoGlgP, CoGBSS/GlgA genes to promote sugar metabolism, decreased the relative expression levels of CoASA, CoTSB, CoPAI, CoTDC and CoCYP71A13 genes for inhibiting IAA biosynthesis and signal transduction, as well as microbial diversity, Fusarium, Albifimbria and Coniosporium abundances in root, ultimately improved the fruit yield of C. oleifera. These findings indicate that intercropping system improves the fruit yield by enhancing the nutrient metabolism capability and crucial microbial abundances in root of C. oleifera in purple soil.

Ubiquitination is the specific modification of target proteins in cells by ubiquitin molecules, which is under the action of a series of special enzymes such as ubiquitin-activating enzymes, binding, and ligase enzymes. Ubiquitination plays an essential role in anthocyanin accumulation in plants. There are few studies on the coloring of pear peel by ubiquitin ligase E3. In this study, an E3 ubiquitin ligase protein PpPUB59 with seven WD40 repeats was cloned. And the function of PpPUB59 on the ubiquitination and protein stability of PpBBX24 and Ppbbx24-del, and the possible action mechanism in the anthocyanin accumulation of 'Red Zaosu' was studied. Our results showed that the WD40 repeats were verified to be the key domain interacting with the VP domain of BBX protein. PpPUB59 could degrade PpBBX24 in vitro by interacting with the VP domain but could not degrade the mutant PpBBX24-del without the VP domain. Dual luciferase assay showed that Ppbbx24-del could activate the PpCHS promoter, while PpPUB59 did not interfere with this activation; PpBBX24 could not activate the promotor of PpCHS but could suppress the activation of PpHY5; when the PpPUB59 was co-expressed with PpBBX24 and PpHY5, the activation roles of PpHY5 in the promotor of PpCHS was not recovered. BiFC and yeast two-hybrid experiments showed that PpPUB59 could also interact with PpHY5, which may make it ubiquitinated and degraded by 26S proteasome. In conclusion, PpPUB59 played an essential role in pear anthocyanin accumulation by ubiquitinating the associated transcription factors. These findings clarified the mutant mechanism of the 'Red Zaosu' pear at the post-translational modification level and enriched the regulation theory of the pear anthocyanin accumulation.

Starch, as the primary storage material in maize endosperm, is essential in determining yield and quality. Although the starch biosynthetic pathway in maize has been well-documented, the transcriptional network underlying endosperm starch synthesis remains elusive. Through a comprehensive co-expression analysis, we screened an endosperm-preferential AP2/ERF transcription factor ZmEREB25, which exhibited a strong correlation with the expression pattern of starch biosynthetic genes in maize endosperm. ZmEREB25 enhanced the promoter activities of the core starch biosynthetic genes, namely Sh2, SSIIIa and SSI, through specific binding to the GCCGAC-containing elements present in their promoters. Given that ZmEREB25 lacked the transactivation capacity, we further identified an ARF transcription factor, ZmARF27, that interacted with ZmEREB25 to coordinately transactivate the promoters of Sh2, SSIIIa and SSI genes via direct binding to these promoters. Our present study demonstrated that the ZmEREB25-ZmARF27 complex is crucial for transactivating core starch synthetic genes in maize endosperm and uncovered a novel regulatory pathway for starch synthesis in maize endosperm.