This article continues our review of spectroscopic studies of G-protein-coupled receptors. Magnetic resonance methods including electron paramagnetic resonance (EPR) and nuclear magnetic resonance (NMR) provide specific structural and dynamical data for the protein in conjunction with optical methods (vibrational, electronic spectroscopy) as discussed in the accompanying article. An additional advantage is the opportunity to explore the receptor proteins in the natural membrane lipid environment. Solid-state H and C NMR methods yield information about the both local structure and dynamics of the cofactor bound to the protein and its light induced changes. Complementary site-directed spin labeling studies monitor the structural alterations over larger distances and correspondingly longer time scales. A multi-scale reaction mechanism describes how local changes of the retinal cofactor unlock the receptor to initiate large-scale conformational changes of rhodopsin. Activation of the G-protein-coupled receptor involves an ensemble of conformational substates within the rhodopsin manifold that characterize the dynamically active receptor.

Here we review the application of modern spectral methods for the study of G-protein-coupled receptors (GPCRs) using rhodopsin as a prototype. Because X-ray analysis gives us immobile snapshots of protein conformations, it is imperative to apply spectroscopic methods for elucidating their function: vibrational (Raman, FTIR), electronic (UV-visible absorption, fluorescence) spectroscopies, and magnetic resonance (electron paramagnetic resonance, EPR), and nuclear magnetic resonance, NMR). In the first of the two companion articles, we discuss the application of optical spectroscopy for studying rhodopsin in a membrane environment. Information is obtained regarding the time-ordered sequence of events in rhodopsin activation. Isomerization of the chromophore and deprotonation of the retinal Schiff base leads to a structural change of the protein involving the motion of helices H5 and H6 in a pH-dependent process. Information is obtained that is unavailable from X-ray crystallography, which can be combined with spectroscopic studies to achieve a more complete understanding of GPCR function.

The study describes an analytical model of laser beam scattering on an inhomogeneous ensemble of erythrocytes. The model takes into account erythrocyte variation in size, shape, and spatial orientation. A relationship is established between an experimentally determined parameter: visibility of the diffraction pattern, and a characteristic serving as a measure of inhomogeneity of erythrocyte size and shape in a blood specimen. This relationship is shown to be monotonous, which means that erythrocyte variation in size and shape can be assessed based on measurements of the diffraction pattern visibility.

The possibility of characterizing fluorescent immunoglobulins using spectrophotometric analysis as a testing method is considered. The comparative analysis of optical properties of fluorescent immunoglobulin preparations and their components-immunoglobulins and fluorochrome-is carried out. The obtained results testify that the proposed methodological approach of optical detection of labeled immunoglobulin molecules can be promising for tests on obtaining conjugates used in immunological tests on revealing specific antigens of causative agents of especially dangerous infections.

The review of recent papers devoted to actively developing methods of photoplethysmographic imaging (the PPGI) of blood volume pulsations in vessels and non-contact two-dimensional oximetry on the surface of a human body has been carried out. The physical fundamentals and technical aspects of the PPGI and oximetry have been considered. The manifold of the physiological parameters available for the analysis by the PPGI method has been shown. The prospects of the PPGI technology have been discussed. The possibilities of non-contact determination of blood oxygen saturation SpO (pulse saturation O) have been described. The relevance of remote determination of the level of oxygenation in connection with the spread of a new coronavirus infection SARS-CoV-2 (COVID-19) has been emphasized. Most of the works under consideration cover the period 2010-2021.