With increasing replacement of native forests with agriculture, it is important to understand the factors allowing non-human primates to persist, including interactions with potential predators. For small-bodied nocturnal primates, smaller carnivores and domestic dogs that often characterise agroforestry landscapes may be a particular threat, especially for primates like slow lorises, which rely on canopy connectivity and are particularly vulnerable on the ground. We present data collected over 12 years in Indonesia. During a long-term study on Javan slow lorises, we used ad libitum sampling to record 703 instances of slow lorises in proximity to wild small carnivores (Javan palm civets, small Indian civets, Sunda leopard cats, Javan ferret badgers) and interactions with small carnivores (62) and domestic dogs (58) respectively. Most of these interactions were neutral or affiliative, including sharing canopy bridges and sleeping sites. Two negative interactions occurred between lorises and wild carnivores; five potential predation events were linked to dogs. Lorises were less active and more vigilant when carnivores were present. A WhatsApp® group facilitated data collection, increasing detail of observations, especially on interactions with domestic dogs. We saw an overall decline in small carnivores, especially nearer to the village, which could be linked to competition with domestic dogs. A better understanding of the role of dogs in this changing landscape is vital.

Individual behavior of primates living in small groups is often seen to represent behavior of all group members due to close spatial cohesion. However, given that females expend more energy on reproduction than males (including lactation and infant carrying), females and males may exhibit different behaviors even when maintaining spatial proximity, particularly in highly seasonal or resource-poor environments. We collected 187 hours of data from three dyads (n = 6 individuals) of white-handed gibbons (Hylobates lar) living in a fruit-poor environment in western Thailand during the period of fruit scarcity. We calculated activity budgets, dyad behavioral synchronization, and dyad spatial cohesion. We hypothesized that activity budgets would differ significantly between sexes or pairs would engage in behaviors independently to provide females with an opportunity to obtain more resources. We also hypothesized that pairs would remain in close proximity. Overall, activity budgets exhibited significant variation when analyzed by sex (X2 = 27.693, P ⩽ 0.001) and group (X2 = 119.584, P ⩽ 0.001). Females spent less time resting and vocalizing and more time traveling compared to males. Percentages of synchronized behavior were lower than expected with only 55% of records synchronized (group B: 58.6%; group D: 58.5%; group L: 49.7%). Spatial cohesion, however, was relatively high overall with adults in the same or adjacent trees in 67.1% of paired records but significantly variable across groups (B: 89.4%; D: 73.1%; L: 48.2%; X2 = 190.111, P ⩽ 0.001). We suggest that behavioral synchronization and spatial cohesion may be indicators of pair bond strength, not just the result of pair living. Given differences in activity budgets, low behavioral synchronization, and variable amounts of time pair mates spent apart, we conclude that pair mates should be considered individual actors who engage in behaviors independently from one another, particularly when coping with challenging ecological conditions.

Opportunities for young people to directly experience nature can have positive impacts on the individual, the social group and on society as a whole, through future increased conservation action. We developed a program which engaged young people from Fort Dauphin in a field trip to see wild animals at Nahampoana Reserve. Our aim in offering field trips to young people living in the city was to create a positive emotional opportunity to engage directly with nature with the ultimate goal of protecting lemurs and their habitat. In our analysis, we predicted that participants who completed a survey after a presentation and field trip (assessment post field trip) would recall more correct and detailed facts than those participants who completed their surveys after the presentation only (assessment pre field trip). Our program used a variety of arts-based data collection methods including an initial essay writing component on conservation of lemurs and drawing analysis of observed plants and animals. Our results show, in part, that (A) initial written essays by participants showed misperceptions about lemurs, (B) participants who had attended the field trip prior to drawing their favourite aspect of the reserve, produced more species-specific plant depictions, and (C) participants who were involved in a presentation, field trip, and final group discussion came up with four principles for protecting lemurs and their environment. Upon analysis after the study, we gained the additional insight of being able to evaluate our survey methods.

Over 91% of the Paraguayan Upper Paraná Atlantic Forest has been cleared and the Gran Chaco is the fastest disappearing habitat on the continent. One of the compounding issues for conservation in Paraguay is the lack of knowledge of the importance of nature amongst the population. Fundación Para La Tierra (PLT) began the country's first Eco-Club program, "Voces de la Naturaleza" (Voices of Nature), in 2016. Three of the curriculums participatory lessons focus on primate conservation. These lessons formed the basis of PLT's Atlantic Forest education programme in 2018 when the team began visiting 22 schools around San Rafael (Tekoha Guasu). To date 26 schools (1,500+ children) across two political departments and four districts have participated in this programme which is now entering its 6th year. The biggest challenges have included the COVID-19 pandemic and the logistics and costs involved in reaching rural and remote schools. Though it is too soon to record observable behavioural change ongoing monitoring over the six year period indicates that children remember the information gained through the participatory methods for several years. We believe that the main reasons for the success of this programme are the collaborations that we have built with the teachers of the schools based on open communication and mutual respect. Though costs can be an issue when beginning, or running, a long-term project we recommend that potential educators not be put off trying and that a lot of good can be achieved with a small amount of funding.

We opportunistically observed 31 cases of responses to sudden loud noises mainly caused by falling rocks and trees in a free-ranging, provisioned group of Japanese macaques, Macaca fuscata, at Katsuyama, Japan, and analyzed 27 cases for which we successfully recorded their responses. In 88% of the 24 cases in which the highest- or second-highest ranking adult male probably heard the noise, one or both of them vocalized aggressively in response. Other males tended to vocalize when they were far away from the two highest-ranking males (in 5 of 7 cases in which they vocalized). Adult females vocalized in only 8% of cases, even when they fled from the noise source. These findings suggest that, unlike tree-shaking and female-directed aggression - both performed often in the mating season and accompanied by vocalizations in both high- and low-ranking males of Japanese macaques - vocalizing in response to sudden loud noises is usually limited to one or two high-ranking males in the vicinity. This vocal response could be a way for the males to advertise their social status; it may also serve to calm group members scared by the noise, preventing them from fleeing in panic and dispersing widely. Our observations also suggest that males may monitor which other males remain nearby, and that whereas females may scream in response to socially induced fear, they are least likely to do so in response to fear arising from non-social contexts.

Environmental education research methods often focus on measuring changes in people's attitudes toward conservation. While attitudes are an important indicator of change, it is critical to target incentivised behaviour because conservation efforts often involve behavioural changes that are costly to one's self (e.g., reducing water use, purchasing pricier alternatives). In the present study, we measured both attitudes and behaviours in a short-term conservation education program near the primate-rich Kibale National Forest in Western Uganda. Adolescents ( N = 59) participated in a three-hour curriculum over three weeks integrated into an after-school wildlife club. After almost 30 years of successful primate conservation education in this region, we chose a novel taxonomic group (bats) for this study. Our pre-registered study had two conditions: an experimental condition, in which students learned about bats as an important local pollinator species, and a control condition, in which students learned about the moon. Only the experimental condition emphasised conservation's delayed benefits. We compared attitudes, knowledge, and behaviours pre- and post- curriculum. To capture behaviour, we employed an investment task in which participants could invest their own resources in a 'fund' to support work relating to bats or the moon, depending on condition. Across both conditions, participants showed positive shifts in attitudes and knowledge from pre- to post-measures and showed increased investments in bat- and moon-related funds. These findings reveal that short-term conservation education influences adolescents' investment decisions as well as their attitudes and knowledge. Our method can easily be edited for conservation education of any species including endangered primates.

People's attitude toward wildlife is known to affect the success of conservation programs. Public education can increase support for conservation measures by providing the knowledge necessary to make informed decisions, improve pro-environmental behaviour and potentially enlist participation. To that end, we developed a participatory conservation education program in Nepal with a target audience of secondary school students. Our lessons emphasized ecological balance by highlighting the roles of individual species, including the rhesus macaque (Macaca mulatta). This species presents a challenge for educating the public about maintaining balance in nature, because although an important seed disperser and species of religious significance, macaques are frequently involved in human-wildlife conflict and considered a pest species. We evaluated the impact of our program by surveying students from six socioeconomically similar schools, four that participated in our program and two that had not. Our questionnaire was comprised of knowledge questions about local wildlife and conservation issues, an open-ended listing exercise to determine species preferences, and statements that elicited students' opinions regarding empathy toward animals. Knowledge about and attitude toward wildlife, including M. mulatta, were greater in students that participated in the program. More importantly, these students were more likely to understand the role individual species play in a balanced ecosystem, how human behaviour can negatively impact wildlife, and how they could participate in conservation efforts. Gender was not a factor in any response. We discuss the merits of using a participatory learning platform in a standalone education program that has long-term purpose, funding, and buy-in.

Chimpanzees (Pan troglodytes) in Senegal may use nocturnality to mitigate hyperthermia risk in semi-arid environments but the degree of nocturnality for such chimpanzees also in sympatry with large carnivores remains uncertain. We compared diel activity among chimpanzees and their potential predators at Assirik in Niokolo-Koba National Park and contextualized these findings relative to other unit-groups in savanna landscapes. From 2015-2018, we generated a predator inventory using multi-modal methods and monitored the diel activity of chimpanzees and predators with camera traps [ N = 2092 camera trap (CT) days]. From 2015-2023, we also surveyed for evidence of predation during recce walks. Six potential nonhuman predators occur at Assirik, including lions (Panthera leo), leopards (Panthera pardus), spotted hyenas (Crocuta crocuta), African wild dogs (Lycaon pictus), Nile crocodiles (Crocodylus niloticus), and rock pythons (Python sebae). We documented one suspected case of a predator killing a chimpanzee. Nocturnality comprised 12.7% of CT events for chimpanzees and these events were more concentrated at twilight. Chimpanzees were more active during the day, predators were more active at night, and there was substantial temporal overlap among chimpanzees and potential predators during twilight intervals. Our findings support the hypothesis that savanna chimpanzees in Senegal are active at night in response to the extremely hot environment. We hypothesize that Assirik chimpanzees experience a tension between decreasing hyperthermia and increasing predation risk during nocturnality.

We aimed to improve knowledge among high school students on local environmental issues, with emphasis on the ecology and threats to primates in the Alto Mayo Valley of San Martin Region, Peru. The Alto Mayo Valley is home to at least 8 species of primate, including the endemic and Critically Endangered San Martín titi monkey (Plecturocebus oenanthe). San Martin is also the most deforested region of Peru. We used two participatory teaching methods: Experiential learning and inquiry-based education. The learning experiences were activities that guided students to look for solutions to real-life situations. The project consisted of 8 lessons co-designed by us and the participating school teachers, and implemented by the same teachers with our guidance. Lesson 5 was a field trip to a local community conservation area to apply primatological field techniques and gather scientific data. The students' final product was a video presentation and a poster of their results. One hundred percent of students acknowledged they learned something new about their local environment, primates, conservation, and research. Experiential learning techniques are effective in developing awareness, knowledge and self-advocacy amongst school students. Our inquiry-based method placed students and teachers closer to scientists, helping them to see the role of science in their neighbourhood. This method can be easily adapted to other regions in Peru and globally.

Introduced species can negatively impact endemic flora and fauna. Studies have primarily utilized camera trap observations and occupancy modelling to better clarify the presence/absence and temporal overlap of endemic and exotic predators. Longitudinal data from field research sites are important as they can provide a finer understanding of predator dynamics and their effects on endemic species. One such site is the Bezà Mahafaly Special Reserve, in southern Madagascar. Protected since the 1970s, the local human population around Bezà Mahafaly Special Reserve has greatly expanded, leading to habitat disturbance in the surrounding forests and increased contact between local wildlife, people and their livestock and dogs. Here we use a combination of scat sampling, field observations of successful and attempted predations, locations of scat samples with identifiable lemur remains, and camera trap data to better assess the predator ecology at Bezà Mahafaly Special Reserve. Our results indicate that forest cats (Felis catus), are effective predators of both adult and infant lemurs and appear to be a constant mammalian predator, utilizing mammal prey more than dogs. Dogs are both predators and scavengers of lemurs. Civets focus on small prey, such as insects and rodents as well as plant material. The fosa, Cryptoprocta ferox, are also present but may not hunt in the area continuously. The killing of an adult ring-tailed lemur by two men from outside the area indicate culturally imposed taboos against lemur killing may no longer be effective given that new arrivals may not share the same local cultural restrictions. Scat sampling of exotic predators is one way to expand our understanding of exotic and endemic predator impact on lemur populations, and long-term studies with multiple assessments of predation can provide a clearer understanding of how non-endemic and endemic predators affect endangered species survival.

Panama is a habitat for nine primate species, and most of them are threatened. This has shown a fundamental concern in primate conservation by Panamanian-based conservation projects. Among them is the project Conservación del Mono Cariblanco en Agroecosistemas (COMOCA) which initiated a primate conservation education program in Southwestern Panama in 2022. The first stage of this program consisted of evaluating the perceptions that 102 children aged 8-12 years old have about primate habitat. I analyzed drawings made by these children exploring three different themes: (1) description of primate species, (2) primate habitat, and (3) food related to primates. The results indicate that children are more familiar with the white-faced capuchins and squirrel monkeys. Despite 89.2% of the children drawing trees, most of them painted tree crops. Thus, the top three most mentioned foods eaten by monkeys were mango, plantain/banana, and apple. These findings highlight the importance of addressing the relationship between primates and native trees in the content of the conservation education program. Additionally, the study illustrates how children's depiction of their reality has implications for the management of human-primate interfaces.

Environmental Education (EE) is key for biodiversity conservation. We aimed to evaluate the effect of an EE activity on students by evaluating their drawings. The EE activity was part of the Blonde Capuchin Project actions, which focuses on the research and conservation of the endangered blonde capuchin monkey, Sapajus flavius. The activity was comprised of four steps and lasted four hours in each school. We analysed 348 drawings made by children from eight schools in rural areas close to forest fragments inhabited by Blonde Capuchins in two municipalities in Northeast Brazil, Igarassu-PE and Mataraca-PB. The children made the drawings during the intervention in each school, specifically in steps 1 and 4 of the activity. We scored the drawings using a rubric to account for the presence of components (biotic and abiotic) and interactions (e.g., human-environment and non-human animal interactions). As an outcome of the EE activity, we found a possible increase in knowledge and awareness through the evaluation of the drawings. Thus, when resources are limited, we encourage the performance of short-term EE activities and the analysis of drawings as a tool to measure their immediate effect.

Adequate knowledge and learning about local biodiversity are a prerequisite for effective attitudinal changes in favour of species protection. Outreach activities are considered a useful tool for sharing information with local stakeholders who play a crucial role in conserving wildlife. We conducted two outreach campaigns focused on schoolchildren in two villages to share information on the natural history of the Bolivian endemic titi monkeys, Plecturocebus olallae and Plecturocebus modestus, to promote their conservation. We assessed the students' ability to retain new information and their understanding of biodiversity through pre- and post-questionnaires, finding an improvement in the knowledge about these two endemic primates from pre- to post-talk assessments, as well as an increase in their awareness about local efforts to preserve biodiversity between outreach campaigns. We also found signals of appropriate experiential learning on wildlife value and its relationship with human activities. Additional outreach work across two decades resulted in important achievements that reflect positive attitudinal changes in favour of the endemic primates and biodiversity, with a remarkable involvement of local people. In this way, we show how outreach work can promote important local support for biodiversity conservation, how primates can act as flagship species, and the need to reinforce knowledge acquisition and learning processes to consolidate conservation actions in the long-term.

Primate males normally protect reproductive females, genetic offspring, and other relatives from external threats. Nevertheless, male protection of group members other than the above individuals is widely reported. Here, we show qualitative data on a silverback's charging behaviors toward human observers (predator surrogates) to protect group members having various age-sex and kinship traits in a group of wild western gorillas containing one reproductive male. We observed 106 and 33 charging behaviors by the leading silverback in two separate study periods. Two natal infants were often involved in his protective charging. Further, the silverback provided protection services to reproductive females. Surprisingly, immigrant individuals (i.e., unrelated to the silverback), including a wide range of age-sex classes, were also protected multiple times. His protection services for natal infants and adult females can be interpreted as a form of parenting effort and mating effort, respectively. Further, those for some immigrant immatures accompanied by their mothers can be considered part of mating effort, advertising his quality as a mate to the mothers. Finally, his charging behaviors to protect immigrant young males, who could be reproductive threats to him, may be due to group augmentation benefits. That is, the recruitment of additional males in exchange for protection services would improve the ability of group defense. Protection services of the leading silverback in the one-male group of western gorillas, in which members of various age-sex classes and kinship traits coexist, could be interpreted by some existing functional explanations.

Environmental education aims to foster knowledge, awareness, and appreciation for nature and can encompass various formats, including festivals. The traveling festival "Changos y Monos va a tu comunidad" is inspired by previous initiatives and aims to promote the conservation of primates in southeast Mexico. The festival involved focused activities, mainly for children, such as talks, games, and exhibitions. It has been held on ten occasions, reaching approximately 700 people from nine localities. Unlike other events, its execution does not require a large budget due to the short duration, the use of small spaces, and because it can be conducted in parallel with field research, which has facilitated its funding. Although no systematic evaluation of the impact of this initiative has been conducted, it is evident that the festival gained notoriety among people in various locations in the region, suggesting "Changos y Monos va a tu comunidad" represents a significant contribution to the conservation of wild Mexican primates.

Conservation education as a key element in fighting species extinction. One of the key objectives of environmental education is to promote pro-environmental behaviours; increasing knowledge and understanding are the first steps. An understanding of the forest and its links to human and wildlife health is essential to foster forest protection. We used NVivo 12 to evaluate a conservation education program based around the Sebangau National Park, Central Kalimantan, Indonesia. Two age groups were evaluated 7-10 years old (37 children) and 11-13 years old (35 children) in 2 sessions conducted approximately 9 months apart. We asked students to write an essay about their knowledge of the forest. Over the 9 months, children increased their use of words accurately associated with BNF activities (0 to 4%), conservation actions (0 to 2%) and forest threats (2 to 12%) as well as expressing more of an emotional connection to the forest and wildlife (0 to 5%). Children who had joined the club for >12 months showed an increase in knowledge about forest characteristics (25 to 30%) and wildlife species names (43 to 55%). While we can demonstrate clear awareness increase on environmental issues, changing mind-sets, attitudes and behaviours does require an in-depth vital experience. We strongly believe that non-formal education is a key complementary tool to inspire new generations to take action towards conservation and sustainable development.

This study explores the perceptions that high school students have about mantled howler monkeys in the Mexican community of Balzapote, Veracruz. We systematized the learning process derived from a Primate Conservation Education Program (PCEP) based on participatory action research (PAR) and arts-based education. Our results indicate a decrease in students' fear of monkeys, the recovery of myths and legends about this species, as well as a complex knowledge about monkeys and their habitat. The inclusion of local knowledge enriched the learning experience and reinforced primate conservation concepts. The PAR and arts-based approaches allowed students to internalize and apply acquired knowledge and fostered empathy. In addition to learning, the approach helped develop teamwork, critical thinking, and creative skills. Quantitative indicators showed shifts in emotions and knowledge post-program, and qualitative data revealed a deep articulation of learned content. We also identified migration and mining activities as socio-political factors impacting local people's livelihoods and wildlife conservation in this region.

The destruction of wildlife habitats due to the exponential growth of human population and its encroachment into wild areas is a major threat to many species around the world. In order to understand the effects of human disturbance on the Philippine tarsier (Carlito syrichta), we evaluated its population status by quantifying the species' population density in an agricultural habitat and assessed the degree of human disturbance in the area. We did the study in Subayon, Bilar, Bohol, where we surveyed 32 ha out of the total 115 ha of the village between April and June 2017. We predominantly used vocal activity to identify the presence and absence of tarsiers, supplementing the data with incidental sightings. In addition, human disturbances were assessed and described qualitatively. We estimated the population density on 75 individuals per square kilometer. The human disturbance was evidenced in 28 out of 32 plots, which indicates that the entire area has already been disturbed. This study showed that the Philippine tarsier could inhabit highly altered habitats such as agricultural landscapes and suggest that Subayon village might be important for the conservation of this species. We provided another example of the ecological flexibility of otherwise specialised nocturnal primates able to inhabit agricultural areas. To avoid conflicts with humans and to conserve such a species in the future, education, outreach and active involvement of local people are essential.

Maderas Rainforest Conservancy (MRC) is a conservation non-profit organization that protects forests, offers primate field schools, and leads community outreach initiatives at sites in Central America, including La Suerte Biological Research Station (LSBRS) in Costa Rica. In 2018, MRC started Aula Verde, a conservation education initiative for elementary school students aged 5-12 at LSBRS. Groups of children from elementary schools and communities located within 50 km have visited LSBRS from 2018-2023 to participate in the 1.5-2 hour Aula Verde workshop, which consists of (1) an interactive lecture on conservation, (2) a guided forest walk, and (3) a snack, gift bag, and tree seedling for each participant. Workshops are funded by international donations to MRC and cost US $8-16 per student. Aula Verde has been successful, with multiple schools and communities making repeat visits, students' families beginning to recycle their garbage and joining a WhatsApp group about conservation education that MRC staff initiated and lead, and teachers reporting improved learning outcomes for students who participate in the workshops. The success of Aula Verde is largely due to the ecological expertise and strong community ties of LSBRS staff. While the COVID-19 pandemic decreased Aula Verde offerings from 2020-2023, we expect workshops to resume at full capacity in 2024. In the future, MRC hopes to expand Aula Verde's outreach to all children aged 5-12 living within 50 km of LSBRS.

Anthropogenic disturbance is a major threat to biodiversity. An anthropogenic disturbance that is rarely addressed for nonhuman primates is the effect of artificial light at night (ALAN) which is defined by the spread of artificial lighting at night which eliminates natural darkness. Artificial light at night can result from streetlights, or indirectly from sky glow (artificial light that is scattered and reflected back to earth by the atmosphere). Research has demonstrated that artificial lighting causes changes in animal behavior, reproductive success, survivorship, as well as can alter the composition of the communities. The goal of this paper is to explore how the behavior of spectral tarsiers, Tarsius spectrumgurskyae, is modified in response to artificial light. We conducted this study at Tangkoko Nature Reserve on the easternmost tip of the northern arm of Sulawesi, Indonesia. The results of this study suggest that in response to artificial light tarsiers increase their time spent travelling and foraging, left their sleeping sites earlier and returned to them later thereby extending their daily activity time. The tarsiers also left their sleeping trees at lower heights, gave fewer vocalizations and fewer alarm calls each night in response to the additional artificial light. The tarsier's lengthened night might be leading to a change in interspecific competition for food as well as increase the ability of potential predators to locate the tarsiers. Additional research on the effect of ALAN on primates is clearly needed.

While there have been recent breakthroughs in human vaginal microbiome research, very few non-human primate (NHP) vaginal microbiome studies exist due to difficulty in obtaining samples. In this study, we sought to: (1) characterize the bonobo vaginal microbiota for the first time, and (2) determine the relationship between vaginal pH and swelling size. During a 21-day study period, we collected observational data and 71 vaginal swabs from three cohoused adult females at the Ape Cognition and Conservation Initiative. After filtering and classification, a total of 3452 amplicon sequence variants were recruited from 12 vaginal samples. The most dominant phylum represented was Actinobacteria, and the most abundant genera were Gardnerella, Atopobium, and Prevotella. The mean pH score was 6.1 (range: 5.1-7.0), and pH levels varied with relative swelling size. This is the first study to examine the vaginal microbial composition in this species, and it conforms to previous NHP studies in that there was not the same bacterial dominance of Lactobacillus spp. often highlighted in human vaginal microbiota studies. Our findings suggest there may be other factors contributing to the protection of the bonobo vaginal environment, but future analysis of a larger sample is needed to fully understand how sociality and sexuality shape vaginal microbiota and host health.