Clouded leopards are notoriously difficult to manage under human care due to their tendency toward high stress, intersex aggression, and limited reproductive success. This case study investigated the endocrinological interplay between adrenal and ovarian steroids and describes a positive correlation between concentrations of fecal estrogen and glucocorticoid metabolites in a female clouded leopard. The female was monitored through two sampling periods approximately 16 months apart, and each data set yielded similar results using a simple linear regression model. The finding of a significant positive correlation between the two steroids represents a novel finding in felids and a hitherto unidentified potential interplay between adrenal and ovarian steroid activity. It also adds to our collective understanding of the effects of estrus on female clouded leopards, impacting conservation efforts as we encourage reproduction in this vulnerable species.

Ozark hellbender (Cryptobranchus alleganiensis bishopi, Grobman 1943) populations in Missouri and Arkansas have been federally listed as endangered since 2011. As part of the comprehensive recovery plan for the subspecies, the Saint Louis Zoo WildCare Institute's Ron and Karen Goellner Center for Hellbender Conservation, in collaboration with the Missouri Department of Conservation, Arkansas Game and Fish Commission, and the U.S. Fish and Wildlife Service, established a conservation breeding and head-starting program to augment and create self-sustaining wild populations. We examined how the river of origin and egg origin (i.e., Zoo-bred or wild-bred) influenced various growth and mortality responses of Zoo-reared Ozark hellbenders. River of origin significantly predicted most larval and long-term Zoo-reared Ozark hellbender growth responses, with our results concurring with observed differences in wild populations and known genetic relationships between Ozark hellbender populations. Mortality of Zoo-reared Ozark hellbenders was often predicted by river of origin and egg origin with Zoo-bred hellbenders having significantly higher proportional mortality responses relative to wild-bred hellbenders. Further exploration of this egg origin relationship revealed differences between the Zoo breeding group generations with higher proportional mortality rates for hellbenders from the less mature second-generation breeding group relative to hellbenders from the first-generation breeding group and wild-bred hellbenders. Ultimately, our results provide baseline data on Zoo-bred and wild-bred Ozark hellbenders in the program, help identify differences in growth and mortality responses between Ozark hellbender populations, and contribute to existing evidence supporting distinct populations of Ozark hellbenders in Missouri to aid in targeted conservation strategies.

Populations of Ozark hellbenders (Cryptobranchus alleganiensis bishopi, Grobman 1943) in Missouri and Arkansas are federally listed as endangered. The Saint Louis Zoo WildCare Institute's Ron and Karen Goellner Center for Hellbender Conservation, in collaboration with the Missouri Department of Conservation and US Fish and Wildlife Service, has developed a sustainable conservation breeding and head-starting program, a priority for species recovery. Using 9 years of program data, we examined various egg production, egg development, and mortality responses of Zoo-bred Ozark hellbenders. Our results identified river of origin and breeding location as important predictors of egg production responses including average breeding female total lengths as well as brooding male clutch size, total egg count, and the estimated number of female clutches, respectively. We found that breeding group generation was a significant predictor of Zoo-bred hellbender egg development responses with hellbenders from the first breeding group generation ovipositing later and producing eggs that hatch later and develop longer than hellbenders from the second-generation breeding group. These responses are consistent with females from the first breeding group generation being larger at the time of reproduction. Breeding group generation was also a significant predictor of proportional egg and total mortality, while the proportion of hatchling mortality was best predicted by breeding location, and the proportion of larval mortality was best predicted by river of origin. Ultimately, our results provide baseline metrics for the program and identify areas for further inquiry to maximize the success of future conservation breeding and head-starting efforts at the Zoo.

Providing nutritionally balanced diets is a key aspect of successful husbandry and can help support sustainable ex situ populations. This study focuses on the diets of zoo-housed colobus monkeys (Colobus angolensis and C. guereza) and langurs (Trachypithecus cristatus and T. francoisi) across AZA institutions. We utilized survey responses from 34 institutions to (1) document the extent of dietary variation with regard to food categories and macronutrients offered, (2) assess interspecific variation in diets offered, and (3) identify important aspects of browse provisioning among institutions. A total of 95 different food items and 229 common-name browse types were offered across institutions. Leafy greens was the only food category offered at every institution. The proportion of root and non-root vegetables in the diet was highly variable. Nonstructural carbohydrates were the most variable macronutrient, and their concentrations were considerably higher than those food items consumed by free-ranging colobines. Conversely, neutral detergent fiber and acid detergent fiber contents were more consistent across institutions but were notably lower than those of free-ranging colobine food items. There were no significant interspecific differences in diets with regard to food categories or macronutrients offered. There was substantial variation in how frequently browse was offered, how it was regarded across zoos (either as diet, enrichment, or both), and whether or how it was quantified. This study provides context for future studies examining whether and how diet and macronutrient intake impact the behavior, health, and welfare of these populations.

Accurate collection of biometric data is important for understanding the biology and conservation of marine organisms, including elasmobranch and teleost fish, both in nature and controlled environments where monitoring marine specimens' health is mandatory. Traditional methods involving specimen capture and handling are invasive, stressful, and disruptive. Some techniques like underwater visual census or laser photogrammetry have been used for noninvasive data collection, but they have limitations and biases. The application of stereo-video photogrammetry through the use of diver-operated stereo-video systems (stereo-DOV) is a noninvasive method that overcomes these challenges, providing highly accurate measurements. It has become popular for species monitoring, studying anthropogenic impacts, and assessing length distributions. However, this technique is still uncommon and barely reported in aquarium settings. This study describes an innovative pilot study targeting multiple species carried out in a Public Aquarium, using a low-cost house-made device. The results revealed that measuring more than 100 individuals in approximately 1 day's work is possible. Total and fork lengths were estimated using specific software for 31 teleost and 16 elasmobranch species and compared with real measurements for the available species. Despite technical limitations that must be reviewed for application in future studies that resulted in high root mean square (RMS) values (> 20 mm), differences between methodological approaches revealed a minimal discrepancy (1.37%-5% in large sharks and rays and 1.8%-5.5% in teleost fish). This technique has time and cost requirements, but might represent a major advance in husbandry and in the contribution to conservation that ex situ studies can provide.

Humans were long thought to be the only mammal to experience menopause, the permanent cessation of reproduction followed by a long post-reproductive lifespan. More recently, evidence has been found for the existence of menopause in other long-lived mammals, including chimpanzees and gorillas. However, orangutans, which have the longest interbirth interval of any primate, have rarely been studied in this period of their lives. In this paper, we describe clinical, ultrasound, endocrine, and histological evidence consistent with a natural menopause in a captive, previously fertile, Sumatran orangutan (Pongo abelii), aged approximately 50. Consecutive serum samples showed low levels of estradiol and high levels of follicle-stimulating hormone. Transvaginal ultrasound revealed an atrophic uterus with an antero-posterior diameter of 2.36 cm, an endometrial thickness of 2 mm, and inactive ovaries. Following this female's death from a subdural hematoma, histological examination of the ovaries showed a dense stroma with corpora albicantia, in comparison to the numerous primordial follicles seen in the ovaries of a stillborn infant female orangutan. These multiple lines of evidence suggest that Sumatran orangutans can now be added to the list of mammals which undergo a true menopause, which may ensure that females' final offspring can be reared to independence.

Amphibians are facing an extinction crisis, with ex situ programs increasingly being used as a tool for their conservation. However, conservation efforts are often limited because we do not understand the ecological, behavioral, and life history traits of many amphibian species. Here, we report on the seasonal breeding patterns, egg-laying behavior, clutch size, and development of 11 frog species maintained at a conservation breeding facility in Andasibe, Madagascar. The frogs exhibited diverse breeding strategies aligned with life history theory. Counting the eggs in 1239 egg masses across these 11 species, we found endotrophic microhylids and terrestrial-breeding species had the smallest clutch size yet completed metamorphosis quickly, whereas species that laid eggs above or in water with exotrophic larvae had larger clutch sizes and took longer to develop. Most reproduction in captivity occurred during the warm, rainy season and followed seasonal patterns in temperature variation. Yet, Mantidactylus betsileanus bred throughout the year, and Heterixalus betsileo required additional environmental stimuli to trigger reproduction. Notably, we confirmed that Gephyromantis mitsinjo lays eggs on land with tadpoles developing terrestrially within jelly, a behavior previously theorized but which until now remained unobserved. Such observations show how captive breeding programs can be used to gain valuable data on the life history traits of species that are otherwise challenging to observe in nature. Our findings can be used to assess threats to closely related species, helping inform conservation efforts in a country harboring exceptional amphibian species richness and endemism.

There is relatively little work examining visitor outcomes of ambassador animal programs, such as whether these programs are educationally effective or unintentionally promote exotic pet ownership. We designed a two-way factorial experiment to test whether ambassador animals enhanced or detracted from the educational outcomes of a zoo exhibit. We surveyed 312 visitors at the Oakland Zoo at a static exhibit focused on the illegal wildlife trade. Participants were surveyed either before or after visiting the exhibit, with or without a trainer presenting either an ambassador ball python (Python regius) or yellow-naped Amazon parrot (Amazona auropalliata). The survey assessed attitudes toward conservation, knowledge of conservation behavior, and the suitability of various species, including the ambassador species, as pets. We used an ANOVA to examine 299 complete surveys for differences between experimental groups' mean responses. There were no significant differences in conservation attitudes. Subjects demonstrated more knowledge of conservation behavior after leaving the exhibit, regardless of whether or not an animal was present. While the exhibit reduced favorable attitudes toward parrots as pets, ambassador animal presence neither positively nor negatively impacted on this effect. Participants who viewed a python were more likely to indicate that they viewed a python as a good pet. However, the sample expressed overall negative attitudes toward exotic pet ownership. This study suggests that ambassadors neither enhanced learning nor unintentionally promoted exotic pets. Further research is needed to understand the role of interpretive messaging when using ambassadors in an educational context.

The secretarybird (Sagittarius serpentarius) is a threatened African raptor species, and as such requires conservation attention. While the species is managed under a European Association of Zoos and Aquaria (EAZA) Ex-situ Programme (EEP), no studies of mortality are currently available. A retrospective mortality project was therefore conducted to identify the most common causes of death in captive secretarybirds, with a view to informing management decisions. Data were collected by requesting the postmortem reports from zoos where secretarybirds had died and combining this with the existing data recorded in the Zoological Information Management System. Overall, 167 birds were recorded as having died within the EEP population, of which post-mortem information was available for 51 (30.54%) individuals. Trauma was the most common cause of death identified (22 birds, 13.17%), with infection appearing as the second most prevalent cause (15 birds, 8.98%). One incidental finding at postmortem was that many (9) birds showed evidence of liver damage, with reports of bacterial hepatitis, staphylococcal infection, lesions, and dark coloration. Further research into liver complications and diet composition for secretarybirds may therefore have value in reducing mortality. Similarly, investigating strategies to reduce trauma caused by other enclosure occupants or conspecifics may have value in lengthening lifespans for this endangered species.

Despite serum ferritin's potential as an iron status indicator, its concentrations vary significantly throughout a black rhinoceros's (Diceros bicornis) life, sometimes irrespective of iron load. We explored acute phase response-related biomarkers, serum amyloid A (SAA) and ceruloplasmin (Cp), to better understand the mechanisms influencing serum ferritin changes in managed black rhinoceroses. The objective was to evaluate the relationships between circulating levels of ferritin, SAA, and Cp in black and white rhinoceroses (Ceratotherium simum). We analyzed banked serum samples collected serially from 11 black (n = 222) and 7 white (n = 134), rhinoceroses (rhinos) and classified samples based on SAA values: clinically healthy (< 1 mg/L), subclinical (1-7 mg/L), or clinically abnormal (> 7 mg/L). In black rhinos, serum ferritin was not different between health status categories (p = 0.5292), nor was it correlated with SAA (p = 0.4164). However, Cp activity was significantly lower in clinically healthy sera (p < 0.0001) and had a moderate positive association with SAA (r = 0.477, p < 0.0001). Among the white rhino samples, only five had SAA values greater than 1 mg/L, limiting the assessment of ferritin and Cp activity in the health context. Minor, yet significant, relationships were observed between serum ferritin and Cp activity, negative in black rhinos (r = -0.206; p = 0.0022) and positive in white rhinos (r = 0.289, p = 0.0008). Cp activity may aid in diagnosing illness in black and white rhinos, based on values observed in sera collected near the time of death. However, acute inflammatory processes do not appear to be one of the primary drivers of the high ferritin concentrations detected in some black rhinos.

"An elephant never forgets" is a popular phrase that refers not only to the elephant's extraordinary ability to remember migration routes but also to its pronounced social long-term memory (SLTM). Previous studies have shown intra- and interspecies SLTM performance, but the ability of elephants to have memories of individual humans has not yet been investigated. We tested this interspecific SLTM using auditory, olfactory, and visual stimuli, each from familiar and unfamiliar persons, in two African savanna elephant (Loxodonta africana) cows living in a zoo. The two-choice object tests revealed a higher interest in sensory stimuli from familiar keepers they had not seen for 13 years than in unfamiliar people. Statistically significant differences were found for olfactory stimuli. In addition, there was significantly more interest in visual stimuli from current keepers than in stimuli from unfamiliar people. Contrary to the results of a previous study with elephants, this was not observed for olfactory stimuli. Due to the small sample size and magnitude of the influencing factors, that is, outdoor experiment, only spatial separation of the animals, these results only represent indications of the possible interspecific SLTM. Nevertheless, we were able to provide the first empirical evidence that L. africana stores information about specific people over a long period of time. Further studies with larger sample sizes, cross-modal testing, and people disliked by the elephants could provide more insights.

Over the past several decades, facultative parthenogenesis (FP)-the ability of a sexually reproducing species to reproduce asexually-in vertebrates has been removed from the realm of obscurity and placed firmly in a position where it warrants focused scientific attention. Likely fueled by increased recognition of the trait, the availability of molecular tools capable of disentangling FP from long-term sperm storage, and the availability of potential cases originating from both zoological and private collections, a wealth of papers has been published revealing the diversity of vertebrate systems in which FP occurs. Specifically, cases have been reported in squamate reptiles (lizards and snakes), crocodiles, birds, and elasmobranch fishes (sharks, rays, and skates). Nonetheless, gaps remain in species documentation, and it is important to analyze and report on new cases. In this paper, we provide a DNA-based analysis confirming FP in a zoo-maintained northern water snake, Nerodia sipedon, a viviparous natricine species that is common and widely distributed in North America. Additionally, we provide information on the sexual development and health of the male parthenogen. Encouragingly, zoological institutions, aquaria, university laboratories, and private collections continue to be rich sources for the further study and documentation of FP in vertebrate species, advancing our understanding of this reproductive trait.

The declining African and Asian elephant populations emphasize the importance of a backup population. Successful reproduction in captivity plays a key role in maintaining such a genetically diverse ex situ population but is challenged by reproductive loss in the form of abortions and stillbirths. The elephants' biphasic prolactin pattern led us to predict a higher incidence of abortions during the time of reduced prolactin concentrations. Therefore, this study focuses on the identification of months during elephant gestation which are prone to loss of pregnancy. A metric was developed to identify the fetal age of aborted calves based on the fetal mass using a regression model. Data on idiopathic abortions in captive and wild elephants collected from zoos, tourist camps, semi-captive, and free-ranging populations since 1974 were analyzed, revealing a significantly higher prevalence of abortions during the 15th and 17th month of gestation. Additionally, the prevalence of stillbirths in the 22nd month of gestation between 2000 and 2023 was assessed. Although stillbirths showed a declining trend over time, the average prevalence between 2019 and 2023 was still 2.8%. Consequently, the 15th, 17th, and 22nd month of gestation were identified as stages prone to pregnancy loss. These findings underscore the necessity of researching risk factors and preventative measures for pregnancy loss in these 3 months, especially exploring a possible link with prolactin during the 15th and 17th month of gestation. The identification of stages prone to fetal loss is a key step towards enhancing elephant reproductive success and welfare.

While housing nonbreeding all-male social groups of African lion (panthera leo) is a necessary part of managing this polygynous species ex situ, resulting intraspecific agonistic behavior can negatively impact animal welfare and guest experience, undermining two primary objectives of modern zoological gardens. Improvest is a gonadotropin releasing factor analog-diphtheria toxoid conjugate marketed for temporary immunological castration which has proven successful in reducing aggression in other zoo-housed species. To the authors' knowledge, the use of this technique has not been described in male African lions; reticence to use GnRH agonists may stem from concern about phenotypic effects (mane loss) and zoo visitor perception. We describe the use of Improvest in conjunction with other management changes to manage agonism in a coalition of African lions (3.0) housed at the Oakland Zoo. Daily agonism scores were calculated via animal care staff records, fecal testosterone levels were measured, and monthly photos were taken to monitor phenotypic changes. While agonism scores varied seasonally in three pretreatment years, a significant (p < 0.05) reduction in the frequency of agonistic behavior began within 2 months of initial treatment. Two lions showed testosterone suppression within 2 months of receiving the first vaccine, while the third showed suppression beginning in the period 4 months after the first vaccine. Mane loss occurred in all three lions, and time to mane regrowth varied between individuals. Improvest, combined with other management strategies, decreased overall fecal testosterone and intraspecific agonism in these lions, and may be an effective tool in other male coalitions.

It has been suggested repeatedly that zoo-kept giant anteaters (Myrmecophaga tridactyla) have higher body masses than their free-ranging conspecifics. Here, we assess this hypothesis by comparing body mass data of 184 female and 173 male individuals kept in zoos to published and unpublished data of free-ranging animals (n = 215). In zoos, the average adult body mass of all individuals was 45.9 ± 9.6 kg (range 19.2-72.6 kg), whereas body mass for free-ranging specimens was 33.0 ± 2.3 kg (21-45.5 kg). Among those zoo animals in which a sufficient number of subsequent intra-annual measures had been taken, we assessed visually whether regular, annual fluctuations in body mass were evident. We observed regular, likely seasonal, cycles in body mass, which (i) may be plausibly attributed to ambient temperature changes that affect the body temperature of anteaters, and (ii) would likely not have occurred if the animals were affected by obesity. Additionally, we explain our hypothesis that in the case of giant anteaters, the discrepancy in body mass between natural habitats and zoos most likely is not indicative of a generally obese population in zoos, as is typically concluded in the case of similar comparisons in primates, but represents an unleashing of the full growth potential of the species by conditions of optimal energy and nutrient provision. Future recording of not only body mass but also body dimensions is recommended to better compare the body condition of free-ranging and zoo-managed individuals.

In Thailand, tigers are more numerous in captivity than they are in the wild, with 51 facilities housing approximately 1962 tigers. As charismatic fauna, tigers are popular with tourists, and the majority of facilities offer a variety of entertainment activities with controversial reports towards the animals' welfare. The aim of this research was to investigate tiger welfare in Thai zoos to identify specific welfare issues. We assessed 34 tourism facilities holding tigers in Thailand in 2019 using a 25-point welfare assessment that utilizes the Five Domain model (incorporating all five domains: nutrition, physical environment, health, behavioural interactions and mental state). The mental domain score was derived from the scores of the other four domains. Additional data were collected from each facility, including the number of tigers, any colour variants, types of human interaction and admission cost. Welfare scores for each domain were calculated by totalling the scores per domain and dividing by the number of points allocated to that domain. A multiple regression was used to identify any significant predictors of mental domain score. The results revealed that the provision of a suitable physical environment scored the lowest, while nutrition scored the highest though this was still a low score overall. The multiple regression showed that 45.4% of the variance for the mental domain score was significantly affected by the number of colour variants housed and the types of human interaction available with facilities, with more of both these factors contributing to a more negative score. Our results demonstrate the need for urgent, comprehensive infrastructural, species-appropriate environment and design and animal management improvements to increase animal welfare. Informed institutional change toward the breeding and use of tigers for public interactions is also required. This is the first assessment completed of captive tiger welfare of tiger facilities in Thailand and shows that the welfare concerns encountered are within a high proportion (67%) of Thailand's facilities. This supports the need for the creation and enforcement of effective and clear captive wildlife operational standards to provide a sustained solution for captive tiger welfare and can provide a considered approach to ex-situ tiger management that, in conjunction with in-situ efforts, can improve much needed conservation efforts of this species.

Zoos are under increasing pressure to strategically manage their collections to maximize visitor attendance, financial income, and their contribution to conservation. As a result, the compositions of zoo collections are undergoing significant changes. Many zoos are keeping fewer species and prioritizing keeping large flagship animals that are more attractive to the public. To understand the effects these changes are having on captive reptile numbers, we have analyzed the trends in reptile holdings between 2003 and 2023 at UK zoos. Our findings show that despite an overall increase in reptile numbers in the period analyzed, there has been a dramatic decline in the number of venomous snakes held at UK zoos, and as a result, venomous snakes are being excluded from many of the conservation benefits that zoos provide. To understand the key factors contributing to the decline in venomous snake numbers, 57 staff members across 35 different BIAZA-accredited zoos were surveyed. Results from the survey identified that a perceived increased risk of harm, increasingly stringent health and safety regulations, and increased husbandry requirements were all key contributing factors to why venomous snake numbers at zoos are in decline.

Conservation breeding programs can save species from extinction by establishing assurance populations that provide individuals for achieving species recovery goals in the wild. However, keeping animals in human care can result in the selection of phenotypes that may be suboptimal for post-release survival and reproduction. It is therefore essential that conservation breeding programs provide animals with naturalistic conditions that facilitate the performance of species-typical behaviors, an endeavor that is also a vital aspect of animal welfare. Here, we describe how we established a conservation breeding program for the 'Akikiki (Oreomystis bairdi), a Hawaiian honeycreeper that is facing imminent extinction in the wild. We share how we utilized the species' native habitat as a template for developing husbandry practices with a primary focus on how we simulate wild environmental conditions in enclosures, mimic naturalistic foraging experiences, and facilitate pair bonding and parental breeding behaviors.

Hand-reared animals are invaluable and irreplaceable in studies of wildlife nutrition. Hand-rearing protocols provide insights into dietary and training programs, but less information is available on disease management. In young ruminants, thiamin (Vitamin B1) deficiency is a particularly important disease that is treatable early in the disease process, but otherwise can be fatal. In this husbandry report, we describe a case of suspected thiamin deficiency in a hand-reared calf (Rangifer tarandus granti) that resulted in clinical signs of polioencephalomalacia and persisted for > 3 months. We attempted treatment with thiamin injections; injections resolved clinical signs of disease, but clinical signs of disease returned once injections stopped. After > 2 months of thiamin injections, the caribou calf received a rumen transfaunation from a fistulated moose (Alces alces) housed at the same facility. Following rumen transfaunation, we did not observe signs of thiamin deficiency. The calf outgrew other females in the cohort initially and shows no long-term effects of thiamin deficiency or polioencephalomalacia. We recommend rumen transfaunation when thiamin deficiency is suspected and does not resolve with thiamin injections alone. We also recommend heterospecific donors if conspecific donors are not available.

For large felids in the wild, intervals between feeding events are irregular since these depend on prey availability and individual hunting success. In contrast, many zoos feed their large felids on fixed schedules. Predictable feeding schedules could induce food anticipatory behavior, like pacing, and randomizing feeding schedules may reduce this anticipatory behavior. Furthermore, random feeding schedules create more variability in the animals environment, which may reduce frustration or boredom. This case study aimed to investigate the effects of a random feeding schedule on pacing behavior of captive Asiatic lions (Panthera leo persica) in the Rotterdam Zoo, The Netherlands. Study animals were observed directly under two treatments: "fixed feeding" (i.e., two fixed feeding days per week) and "random feeding" (i.e., two semi-randomly picked feeding days per week). Under the random feeding schedule, the mean pacing proportion of lions significantly decreased compared to the fixed feeding schedule. These findings suggest that a random feeding schedule could reduce pacing, either of anticipatory or stereotypic nature, in Asiatic lions. Random feeding schedules do pose a few logistic challenges, such as zookeeper schedules and transport or storage of feed. Nevertheless, random feeding could be a relatively inexpensive strategy to reduce pacing.

Providing evidence-based care to animals is a common goal in zoos and aquariums. However, there are many understudied species with a lack of information available, which may result in suboptimal care for individuals of these species. Using preference tests to ask individual animals what they want can be an effective means to enhance their care, and subsequently, their welfare. Using 24-h remote monitoring, we assessed substrate, enclosure feature, and lighting preferences of five aquatic turtles (two wood turtles, one Blanding's turtle, and two spotted turtles) that share an indoor enclosure at Lincoln Park Zoo. In addition, we compared enclosure feature and lighting preferences between winter and summer to determine whether the turtles had changing seasonal preferences despite being housed indoors. We found that the wood turtles and Blanding's turtle had substrate preferences, whereas the spotted turtles did not. In addition, we found that the turtles' enclosure feature preferences were consistent between winter and summer, but the amount of time spent on land, logs, and in water varied by individual. Finally, we found that the turtles utilized areas with ambient lighting more than areas with a heat lamp or UV lamp; this was consistent between winter and summer. The turtle preferences observed in this study can contribute to the development of care strategies that support their welfare and, in turn, may inspire similar preference studies at other institutions as we collectively advance toward providing animals of all taxonomic groups with evidence-based care.