Genetic seascape of the threatened Caribbean elkhorn coral, , on the Puerto Rico Shelf
It has been proposed that the elkhorn coral, , is genetically separated into two distinct provinces in the Caribbean, an Eastern and a Western population admixing in western Puerto Rico and around the Mona Passage. In this study, the genetic structure of sampled at 11 Puerto Rican localities and localities from Curaçao, the Bahamas and Guadeloupe were examined. Analyses using five microsatellite markers showed that 75% of sampled colonies had unique genotypes, the rest being clone mates. Genetic diversity among genets was high (H = 0.761) and consistent across localities (0.685 to 0.844). ranged from -0.011 to 0.047 supporting low but significant genetic differentiation between localities within the previously reported Eastern and Western genetic provinces. Plots of genetic per geographic distances and significant Mantel tests supported isolation-by-distance (IBD) within Puerto Rico. Analysis with the software favored a scenario with weak differentiation between two populations, assigning eastern Puerto Rican locations (Fajardo and Culebra), Guadeloupe and Curaçao to the Caribbean Eastern population and western Puerto Rican locations (west of Vega Baja and Ponce), Mona and the Bahamas to the Caribbean Western population. Vieques and San Juan area harbored admixed profiles. Standardized s per 1,000 km unit further supported higher differentiation between localities belonging to different populations, with IBD being stronger within Puerto Rico than on larger regional scales. This stronger genetic transition seems to separate localities between putative Eastern and Western provinces in the eastern Puerto Rican region, not around the Mona Passage.
Skin toxins in coral-associated species (Teleostei: Gobiidae) affect predator preference and prey survival
Predation risk is high for the many small coral reef fishes, requiring successful sheltering or other predator defence mechanisms. Coral-dwelling gobies of the genus live in close association with scleractinian corals of the genus . Earlier studies indicated that the low movement frequency of adult fishes and the development of skin toxins (crinotoxicity) are predation avoidance mechanisms. Although past experiments showed that predators refuse food prepared with goby skin mucus, direct predator-prey interactions have not been studied. The present study compares the toxicity levels of two crinotoxic coral gobies - , representative of a conspicuously coloured species, and sp.3 with cryptic coloration - using a standard bioassay method. The results show that toxin levels of both species differ significantly shortly after mucus release but become similar over time. Predator preferences were tested experimentally in an aquarium in which the two gobies and a juvenile damselfish were exposed to the small grouper . Video-analysis revealed that although coral gobies are potential prey, clearly preferred the non-toxic control fish () over . When targeting a goby, the predator did not prefer one species over the other. Contrary to our expectations that toxic gobies are generally avoided, gobies were often captured, but they were expelled quickly, repeatedly and alive. This unusual post-capture avoidance confirms that these gobies have a very good chance of surviving attacks in the field due to their skin toxins. Nonetheless, some gobies were consumed: the coral shelter may therefore also provide additional protection, with toxins protecting them mainly during movement between corals. In summary, chemical deterrence by crinotoxic fishes seems to be far more efficient in predation avoidance than in physical deterrence involving body squamation and/or strong fin spines.
Analysis of Benthic Communities in the Cyclades Plateau (Aegean Sea) Using Ecological and Paleoecological Data Sets
In the Cyclades plateau (Aegean Sea), a qualitative and quantitative analysis of macro-benthic fauna was carried out in 1986. Standard multivariate analysis techniques were applied to both ecological (living benthic fauna) and paleoecological data sets in order to distinguish distribution patterns. Results showed that caution must prevail in drawing conclusions from a limited data set. The clearest classification was obtained using total living fauna, while the dead molluscan fauna gave a similar pattern; this indicates similar response to the environmental conditions of the area. In the analysis of the living molluscan fauna, the groups failed to show any clusters, probably as an effect of some impoverished sites. In the two groups delineated, depth seems to be the major factor in the distribution of species. The fact that two distinct data sets (subfossil assemblages and living communities), when treated separately, produce similar grouping indicates that the subfossil assemblages could be reliably used as a first approach for determination of the living communities' distribution patterns.